HUMAN SUBMANDIBULAR-SUBLINGUAL SALIVA PROMOTES ADHESION OF CANDIDA-ALBICANS TO POLYMETHYLMETHACRYLATE
HUMAN SUBMANDIBULAR-SUBLINGUAL SALIVA PROMOTES ADHESION OF CANDIDA-ALBICANS TO POLYMETHYLMETHACRYLATE
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DOI：
10.1128/iai.61.6.2644-2652.1993
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发表时间：
1993-06-01
影响因子：
3.1
通讯作者：
LEVINE, MJ
中科院分区：
文献类型：
--
作者：
EDGERTON, M;SCANNAPIECO, FA;LEVINE, MJ
这项研究的目的是确定唾液中与白色念珠菌在溶液中相互作用的成分，以及可能调节与牙科丙烯酸(聚甲基丙烯酸甲酯[PMMA])表面粘附性的成分。从白色念珠菌芽生孢子和菌丝状细胞中提取的唾液膜和新鲜的人颌下舌下唾液(HSMSL)混合后，主要含有高分子粘蛋白(MG1)和低分子粘蛋白(MG2)。相比之下，鲜人腮腺唾液中很少的成分被酵母细胞吸附。与人腮腺唾液相比，涂有HSMSL的PMMA微珠显著增强(10倍)两种生长形式的白色念珠菌的粘附性(2倍)，这表明粘蛋白在粘附性中的作用。在涂覆PMMA之前，用胚生孢子或菌丝状细胞预吸附HSMSL，可完全消除HSMSL增强的粘附力。然而，用纯化的唾液粘蛋白包被PMMA或在预吸附的唾液中添加粘蛋白并不能增强或恢复粘附性到新鲜HSMSL的水平。使用胍处理的新鲜HSMSL进行的黏附分析显示，完全缺乏念珠菌的结合，这表明HSMSL处于解离条件下可能会改变唾液粘蛋白的一种对白念珠菌黏附至关重要的性质。酵母菌的蛋白酶和糖苷酶处理显著降低了与HSMSL包被的PMMA的粘附性。此外，白念珠菌与甘露糖和半乳糖预先孵育可抑制与HSMSL包被的PMMA的粘附性。这些结果表明，粘蛋白可能在白色念珠菌与唾液包裹的PMMA之间的黏附中发挥作用，酵母表面的糖蛋白可能参与了这些事件。
The purpose of this study was to identify components of saliva that interact with Candida albicans in solution and that may modulate adhesion to dental acrylic (polymethylmethacrylate [PMMA]) surfaces. Saliva-derived pellicles extracted from C. albicans blastoconidia and hyphal-form cells mixed with fresh human submandibular-sublingual saliva (HSMSL) contained predominantly high- and low-molecular-weight mucins (MG1 and MG2, respectively). In contrast, few components from fresh human parotid saliva were adsorbed to yeast cells. Coating PMMA beads with HSMSL significantly enhanced (10-fold) adhesion of both growth forms of C. albicans compared with human parotid saliva (2-fold), suggesting a role for mucins in adhesion. HSMSL-enhanced adhesion was completely abolished by preadsorbing HSMSL with either blastoconidia or hyphal-form cells prior to coating PMMA. However, coating PMMA with purified salivary mucins or the addition of mucin to preadsorbed saliva did not enhance or restore adhesion to levels found with fresh HSMSL. Adhesion assays employing guanidine-treated fresh HSMSL showed a complete lack of Candida binding, suggesting that subjecting HSMSL to dissociating conditions may alter a property of salivary mucins crucial for C. albicans adhesion. Protease and glycosidase treatment of yeast cells significantly reduced adhesion to HSMSL-coated PMMA. In addition, preincubation of C. albicans with mannose and galactose inhibited adhesion to HSMSL-coated PMMA. These results suggest that mucins may play a role in C. albicans adhesion to saliva-coated PMMA and that a glycoprotein on the yeast surface may be involved in these events.