Characterization of changes of pain behavior and signal transduction system in food-deprived mice.
Characterization of changes of pain behavior and signal transduction system in food-deprived mice.
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DOI：
10.1080/19768354.2018.1490348
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发表时间：
2018
影响因子：
2.9
通讯作者：
Suh HW
中科院分区：
文献类型：
--
作者：
Jang SP;Park SH;Jung JS;Lee HJ;Hong JW;Lee JY;Suh HW
禁食通常会引起几种代谢变化。在本研究中，我们研究了几种类型的伤害性感受在食物剥夺过程中的可能变化。强迫小鼠禁食12、24、48 h后，采用甩尾、扭体、福尔马林、von-frey等方法检测小鼠伤害性感受的变化。 我们发现，腹腔注射（i. p.）禁食组中给予醋酸（扭体反应）或后爪足底注射5%福尔马林的反应减少。此外，禁食组甩尾反应和机械von-frey试验的伤害性感受阈值也升高。此外，在背根神经节（DRG）和脊髓中的p-CREB和p-ERK水平在食物剥夺组中降低。此外，禁食组大鼠背根节和脊髓p-AMPKα1表达上调，而背根节和脊髓p-mTOR表达下调。我们的研究结果表明，化学，机械和热伤害性感受似乎减少了食物剥夺小鼠组。此外，缺食组伤害性感受的降低与DRG和脊髓中ERK、CREB、AMPKα1和mTOR等信号转导分子的表达密切相关。
Fasting in general causes several metabolic changes. In the present study, we examined the possible changes of several types of nociception during the food deprivation were investigated in mice. After the mice were forced into the fasting for 12, 24, or 48 h, the changes of nociception were measured by the tail-flick, writhing, formalin or von-frey tests. We found that the nociceptive behavior induced by intraperitoneally (i.p.) administered acetic acid (writhing response) or intraplantar injection of 5% formalin into the hind-paw were reduced in fasted group. In addition, the tail-flick response and threshold for nociception in mechanical von-frey test were also elevated in fasted group. Moreover, the p-CREB and p-ERK levels in the dorsal root ganglia (DRG) and the spinal cord were reduced in food-deprived group. Furthermore, p-AMPKα1 expressions in DRG and the spinal cord were up-regulated, whereas p-mTOR in DRG and the spinal cord was down-regulated in food-deprived group. Our results suggest that the chemical, mechanical, and thermal nociceptions appear to be reduced in a food-deprived mouse group. Additionally, reduction of nociception in food-deprived group appears to be closely associated with the expressions of several signal transduction molecules such as ERK, CREB, AMPKα1 and mTOR proteins in DRG and the spinal cord.