INITIATION OF ENDOCHONDRAL CALCIFICATION IS RELATED TO CHANGES IN THE REDOX STATE OF HYPERTROPHIC CHONDROCYTES
INITIATION OF ENDOCHONDRAL CALCIFICATION IS RELATED TO CHANGES IN THE REDOX STATE OF HYPERTROPHIC CHONDROCYTES
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DOI：
10.1126/science.7112108
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
FRASCA, P
中科院分区：
文献类型：
--
作者：
SHAPIRO, IM;GOLUB, EE;FRASCA, P
用高分辨扫描显微荧光法和生化分析法测定了鸡矿化生长板中吡啶核苷酸（NADH和NAD+）的含量。扫描电子显微镜和光学显微镜被用来与软骨细胞成熟的阶段的NADH和NAD+的浓度。一个显着的增加，发现在肥大区的还原吡啶核苷酸的相对浓度。在该区域的两侧，在增殖和钙化的软骨中，NADH荧光减少，并且NADH/NAD+比率被抑制。与骨矿物质的最早沉积相关的组织区中的NADH积累支持氧化还原状态的变化启动组织矿化的假设。
The level of pyridine nucleotides (NADH and NAD+) in the mineralizing growth plate of the chick was ascertained by high-resolution scanning microfluorimetry and biochemical analysis. Scanning electron microscopy and light microscopy were used to relate the concentrations of NADH and NAD+ to stages of chondrocyte maturation. A dramatic increase was found in the relative concentration of reduced pyridine nucleotides in the hypertrophic zone. On either side of this zone, in proliferating and calcifying cartilage, there was a decrease in NADH fluorescence, and the NADH/NAD+ ratio was depressed. NADH accumulation in the tissue zone associated with the earliest deposition of bone mineral supports the hypothesis that a change in the redox state initiates tissue mineralization.