Immunoelectron microscopic single and double labelling of aminopeptidase N (CD 13) and dipeptidyl peptidase IV (CD 26)
Immunoelectron microscopic single and double labelling of aminopeptidase N (CD 13) and dipeptidyl peptidase IV (CD 26)
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DOI：
10.1016/s0065-1281(96)80025-0
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发表时间：
1996-07-01
影响因子：
2.5
通讯作者：
Holzhausen, HJ
中科院分区：
文献类型：
--
作者：
Stange, T;Kettmann, U;Holzhausen, HJ
用超薄冰冻切片和免疫金标记法，将氨基肽酶N(CD13)和二肽基肽酶IV(CD26)定位于人肾近端小管细胞和大鼠小肠肠上皮细胞刷状缘膜的管腔侧。此外，这两种酶均可在人T淋巴细胞细胞表面检测到，尤其是在人滑膜成纤维细胞上检测到氨基肽酶N。近端肾小管上皮细胞和滑膜成纤维细胞的胞浆内也可见金标记的泡状结构。在人肾中，通过几种双重标记方法，两种膜抗原的共存成为可能。
Using ultrathin cryosections and immunogold labelling, aminopeptidase N (CD 13) and dipeptidyl peptidase IV (CD 26) were localized on the luminal side of the brush border membrane of proximal tubular cells in human kidney as well as of enterocytes from rat small intestine. Furthermore, both enzymes could be detected on the cell surface of human T lymphocytes and especially aminopeptidase N on human synovial fibroblasts. Gold labelled vesicular structures were also found in the cytoplasm in the apical part of renal proximal tubular cells and synovial fibroblasts. In human kidney the colocalization of the two membrane antigens was possible by using several double labelling methods.