Antagonism of alcohol-induced suppression of rat testosterone secretion by an inhibitor of nitric oxide synthase.
Antagonism of alcohol-induced suppression of rat testosterone secretion by an inhibitor of nitric oxide synthase.
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一氧化氮合酶抑制剂对抗酒精引起的大鼠睾酮分泌抑制。
DOI：
10.1111/j.1530-0277.1993.tb00815.x
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Cicero,TJ
中科院分区：
文献类型：
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作者：
Adams,ML;Forman,JB;Kalicki,JM;Meyer,ER;Sewing,B;Cicero,TJ
为了检查一氧化氮（NO）是否介导酒精（乙醇）抑制睾酮分泌，用一氧化氮合酶抑制剂NG-硝基-L-精氨酸甲酯（NAME）预处理成年雄性大鼠，然后用酒精治疗。酒精治疗后 2 小时，即酒精和 NAME 对睾酮分泌的影响达到峰值时，测量血清和睾丸间质液 (TIF) 睾酮浓度、血清黄体激素 (LH) 浓度、血液酒精浓度 (BAC) 和 TIF 体积。在酒精治疗（0.5-3 g/kg，腹腔内）前 30 分钟用 NAME（30 或 100 mg/kg，皮下注射）进行预处理，完全阻断了酒精引起的睾酮分泌到大循环和 TIF 中的抑制，而不显着改变血液酒精浓度 (BAC) 或 TIF 体积。这些结果支持这样的假设：一氧化氮合酶抑制剂可以拮抗酒精诱导的睾丸类固醇生成抑制，酒精与调节睾丸类固醇生成的精氨酸-一氧化氮合酶系统相互作用，以及一氧化氮参与介导酒精对睾丸和生殖的影响。
To examine whether nitric oxide (NO) mediates the suppression of testosterone secretion by alcohol (ethanol), adult male rats were pretreated with a NO synthase inhibitor, NG‐nitro‐L‐arginine methyl ester (NAME), then treated with alcohol. Serum and testicular interstitial fluid (TIF) testosterone concentrations, serum luteiniring hormone (LH) concentrations, blood alcohol concentrations (BAC), and TIF volumes were measured 2 hr after alcohol treatment at a time of peak effects of alcohol and NAME on testosterone secretion. Pretreatment with NAME (30 or 100 mg/kg, subcutaneous) 30 min before alcohol treatment (0.5–3 g/kg, intraperitoneal) completely blocked the alcohol‐induced suppression of testosterone secretion into the general circulation and into TIF without significantly altering blood alcohol concentrations (BAC) or TIF volumes. These results support the hypotheses that NO synthase inhibitors can antagonize alcohol induced suppression of testicular steroidogenesis, that alcohol interacts with arginine‐NO synthase systems that regulate testicular steroidogenesis, and that NO is involved in mediating alcohol's testicular and reproductive effects.