Magnetic engineering of stable rod-shaped stem cell aggregates: circumventing the pitfall of self-bending.
Magnetic engineering of stable rod-shaped stem cell aggregates: circumventing the pitfall of self-bending.
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DOI：
10.1039/c4ib00219a
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发表时间：
2015-02
期刊：
影响因子：
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通讯作者：
V. Du;D. Fayol;M. Reffay;N. Luciani;J. Bacri;C. Gay;C. Wilhelm
中科院分区：
文献类型：
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作者：
V. Du;D. Fayol;M. Reffay;N. Luciani;J. Bacri;C. Gay;C. Wilhelm
在恢复病变或受损组织的功能时，组织工程目前面临的一个挑战是根据目标区域定制组织。无支架入路通常形成球形，由于营养和氧气扩散不良，有中心坏死的风险。在这里，我们利用微型磁铁在细胞尺度上产生的磁力来创造干细胞的棒状聚集体，这些干细胞随后成熟为组织样结构。然而，在成熟过程中，组织杆自发地弯曲和盘绕成球形结构，这是由最初不均匀的组织中细胞-细胞黏附增加引起的。组织内磁力的优化成功地阻碍了这种转变，以产生稳定的杆状干细胞聚集体。
A current challenge for tissue engineering while restoring the function of diseased or damaged tissue is to customize the tissue according to the target area. Scaffold-free approaches usually yield spheroid shapes with the risk of necrosis at the center due to poor nutrient and oxygen diffusion. Here, we used magnetic forces developed at the cellular scale by miniaturized magnets to create rod-shaped aggregates of stem cells that subsequently matured into a tissue-like structure. However, during the maturation process, the tissue-rods spontaneously bent and coiled into sphere-like structures, triggered by the increasing cell-cell adhesion within the initially non-homogeneous tissue. Optimisation of the intra-tissular magnetic forces successfully hindered the transition, in order to produce stable rod-shaped stem cells aggregates.