Three-dimensional photolithographic patterning of multiple bioactive ligands in poly(ethylene glycol) hydrogels
Three-dimensional photolithographic patterning of multiple bioactive ligands in poly(ethylene glycol) hydrogels
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DOI：
10.1039/c0sm00140f
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发表时间：
2010-01-01
期刊：
影响因子：
3.4
通讯作者：
West, Jennifer L.
中科院分区：
文献类型：
--
作者：
Hoffmann, Joseph C.;West, Jennifer L.
生物材料界面临着模拟极其复杂的生理组织环境的挑战。虽然传统上已经在二维（2D）中研究了为此目的的细胞系统，但大多数细胞需要三维（3D）线索来产生生理相关的响应。双光子吸收激光扫描光刻（TPA-LSL）可应用于光敏水凝胶系统，以设计由精确图案化的生物活性信号组成的异质3D微环境。在这项工作中，我们已经开发了新的操作参数和系统功能的TPA-LSL通过图案化的荧光标记的单丙烯酸酯PEG-RGDS在PEG-DA水凝胶。具体而言，我们已经证明了一个灵活的图案尺寸范围内，从1毫米到近1毫米的功能。我们还显示了不同浓度的细胞粘附配体RGDS和相关的RGDS荧光与激光扫描速度和强度的模式。最后，我们将多个独特的生物活性配体微图案化为单个水凝胶内的独特的3D形式。这里提出的结果显着开发的TPA-LSL微图案化技术的能力，允许制造异质性，三维细胞微环境，这应该证明是非常有用的未来的仿生应用。
The biomaterials community is faced with the challenge of imitating a vastly complex, physiological tissue environment. While cellular systems towards this end have been traditionally studied in two dimensions (2D), most cells require three-dimensional (3D) cues to produce a physiologically relevant response. Two-photon absorption laser scanning lithography (TPA-LSL) may be applied to photosensitive hydrogel systems to engineer heterogeneous, 3D microenvironments consisting of precisely patterned bioactive signals. In this work, we have developed new operating parameters and system capabilities for TPA-LSL through the patterning of fluorescently labeled monoacrylate PEG-RGDS within PEG-DA hydrogels. Specifically, we have demonstrated a flexible pattern size range, with features ranging from 1 mm to nearly 1 mm. We have also shown patterns of differing concentrations of the cell adhesive ligand RGDS and correlated observed RGDS fluorescence with laser scan speed and intensity. Finally, we have micropatterned multiple, unique bioactive ligands into distinct, 3D forms within a single hydrogel. The results presented here have significantly developed the capabilities of the TPA-LSL micropatterning technique to allow for the fabrication of heterogeneous, 3D cellular microenvironments, which should prove highly useful for future biomimetic applications.