Layer-by-layer self-assembly of polyelectrolyte multilayers on silica spheres as reversed-phase/hydrophilic interaction mixed-mode stationary phases for high performance liquid chromatography
Layer-by-layer self-assembly of polyelectrolyte multilayers on silica spheres as reversed-phase/hydrophilic interaction mixed-mode stationary phases for high performance liquid chromatography
复制标题
二氧化硅球上聚电解质多层的层层自组装作为高效液相色谱的反相/亲水相互作用混合模式固定相
DOI：
10.1016/j.chroma.2017.03.083
复制
发表时间：
2017
影响因子：
4.1
通讯作者：
Zhang Shusheng
中科院分区：
文献类型：
--
作者：
Zhao Wenjie;Jiang Xiuming;Ni Shujiao;Guo Yun;He Lijun;Xiang Guoqiang;Zhang Shusheng
在这项研究中，通过逐层自组装方法制备了一系列固定相，包括分别作为核心和表面涂层的二氧化硅球和聚电解质多层膜（PEM），用于高效液相色谱。通过聚（乙烯-马来酸酐-alt）与正烷基醇（CnH 2n +1 OH，n = 4，8，10，12）的酯化反应，合成了带正电荷的聚（烯丙胺盐酸盐）和带负电荷的聚（乙烯-马来酸酐-alt）阴离子，并将其交替沉积在质子交换膜上。以烷基苯、对羟基苯甲酸及其酯类、苯胺类、酚类和嘧啶类为探针，采用反相/亲水作用混合模式色谱，对新型固定相的色谱性能和保留机理进行了评价。通过调节聚（乙撑-马来酸酯-马来酸酯）的链长和聚合物膜的双层层数，优化了新型固定相的分离性能。这些结果表明，新的固定相表现出的承诺，同时分离复杂的疏水性和亲水性样品具有高选择性。
In this study, a series of stationary phases, comprising silica spheres and polyelectrolyte multilayers (PEMs) as the core and surface coating, respectively, were prepared via a layer-by-layer self-assembly approach for use in high-performance liquid chromatography. PEMs coatings were formed by the alternate deposition of positively charged poly(allylamine hydrochloride) and negatively charged poly(ethylene-alt-maleate) anions which were synthesized by esterification between poly(ethylene-alt-maleic anhydride) andn-alkyl alcohols (CnH2n+1OH, n = 4, 8, 10, 12). The chromatographic performance and retention mechanism of the new stationary phases were evaluated in simultaneous reversed-phase/hydrophilic interaction mixed-mode chromatography using different solute probes, such as alkyl benzene,p-hydroxybenzoic acid and its esters, anilines, phenols, and pyrimidines. The separation performance of the new stationary phases was optimized by tuning then-alkyl chain length of poly(ethylene-alt-maleate) and bilayer numbers of PEMs. These results indicated that the new stationary phases demonstrate promise for the simultaneous separation of complex hydrophobic and hydrophilic samples with high selectivity.