Age-related alteration of neurturin receptor GFRa2 and nNOS in pelvic ganglia
Age-related alteration of neurturin receptor GFRa2 and nNOS in pelvic ganglia
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DOI：
10.1016/j.neurobiolaging.2005.07.016
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发表时间：
2006-10-01
影响因子：
4.2
通讯作者：
Tsukamoto, Taiji
中科院分区：
文献类型：
--
作者：
Hisasue, Shin-ichi;Kato, Ryuichi;Tsukamoto, Taiji
Neurturin是一种神经营养因子，广泛表达于海绵体组织中，并通过其受体胶质细胞系衍生的神经营养因子家族受体α-2（GFRa 2）逆行转运至阴茎投射神经元。为了探讨衰老对阴茎神经功能的影响，我们检测了GFRa 2 mRNA在盆大神经节中的表达及其与神经元型一氧化氮合酶（nNOS）和酪氨酸羟化酶（TH）阳性神经元的关系。RT-PCR结果显示GFRa 2和nNOS mRNA表达水平在1、3、6、12、18和24月龄大鼠中随年龄增长而降低。原位杂交结果还显示，随着年龄的增长，盆神经节中GFRa 2阳性神经元的数量减少。双标记研究显示GFRa 2和nNOS的共表达，与年轻成年大鼠（3个月）相比，老年成年大鼠（24个月）和年轻去势大鼠中GFRa 2和nNOS的共表达同时降低。这些结果表明，老化和去势影响nNOS和GFRa 2阳性神经元的数量。年龄的增加不仅影响海绵体组织，而且影响与勃起功能相关的海绵体神经的可塑性。(c)2005年爱思唯尔公司All rights reserved.
Neurturin is a neurotrophic factor that is widely expressed in cavernous tissue and retrogradely transported to penis-projecting neurons via its receptor, glial cell line derived neurotrophic factor family receptor alpha-2 (GFRa2). To investigate the influence of aging on neural function on the penis, we examined the expression of GFRa2 mRNA in the major pelvic ganglion and its relationship to neuronal nitric oxide synthase (nNOS)- and tyrosine hydroxylase (TH)-positive neurons. GFRa2 and nNOS mRNA expression levels in RT-PCR showed age-related decreases in 1-, 3-, 6-, 12-, 18- and 24-month-old rats. In situ hybridization also revealed that the number of GFRa2-positive neurons in pelvic ganglia decreased with aging. A double-labeling study revealed the co-expression of GFRa2 and nNOS, which simultaneously decreased in old adult (24 months) and young castrated rats compared with young adult rats (3 months). These results suggest that aging and castration influence the numbers of nNOS- and GFRa2-positive neurons. Higher age might affect not only cavernous tissue but also the neural plasticity of the cavernous nerve related to erectile function. (c) 2005 Elsevier Inc. All rights reserved.