Mini-Sheet Injection for Cultured Corneal Endothelial Transplantation
Mini-Sheet Injection for Cultured Corneal Endothelial Transplantation
复制标题
用于培养角膜内皮移植的微型片注射
DOI：
10.1089/ten.tec.2018.0077
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发表时间：
2018-08-01
影响因子：
3
通讯作者：
Shi, Weiyun
中科院分区：
文献类型：
--
作者：
Jia, Yanni;Li, Wenjing;Shi, Weiyun
角膜内皮是单层六角形细胞，通过其屏障和泵功能维持角膜的透明度和厚度。针对角膜内皮功能障碍的治疗，由于全球供体角膜严重短缺，组织工程角膜内皮移植和直接注射培养角膜内皮细胞的技术应运而生。但技术难度或安全风险依然存在。在本研究中，我们报道了一种用于培养角膜内皮细胞移植的新型迷你片注射方法，并与兔模型中单细胞注射的效果进行了比较。与报道的单细胞注射相比，迷你片促进了注射后的粘附和紧密连接的形成。与单细胞注射 14 天相比，迷你片注射 7 天后，兔角膜透明度和厚度迅速恢复。此外，早在迷你片注射后7天就观察到了典型的内皮形态，而直到单细胞注射后21天才观察到典型的内皮形态。这些结果表明，新型小片注射角膜内皮细胞表现出快速粘附、紧密连接形成和角膜透明度恢复，这可能代表了一种更有效的培养角膜内皮细胞移植方法。
Corneal endothelium is a single layer of hexagonal cells that maintains the corneal transparency and thickness through its barrier and pump function. For the treatment of corneal endothelial dysfunction, the transplantation of tissue-engineered corneal endothelium and direct injection of cultured corneal endothelial cells were developed because of the severe shortage of donor cornea worldwide. However, the technique difficulty or safety risk still remained. In this study, we report a novel mini-sheet injection for the cultured corneal endothelial cell transplantation and compare with the effects of single-cell injection in rabbit model. Compared with the reported single-cell injection, mini sheets promoted the adhesion and tight junction formation after injection. Rabbit corneal clarity and thickness were rapidly recovered after 7 days of mini-sheet injection, compared with 14 days of single-cell injection. Moreover, typical endothelial morphology was observed as early as 7 days after the mini-sheet injection, whereas until 21 days after the single-cell injection. These results demonstrate that the novel mini-sheet injection of corneal endothelial cells exhibited rapid adhesion, tight junction formation, and corneal clarity recovery, which may represent a more efficient method for the transplantation of cultured corneal endothelial cells.