Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticus
Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticus
复制标题
基于酶的比色适体传感器检测副溶血弧菌
DOI：
10.1021/acs.jafc.5b03224
复制
发表时间：
2015-09-09
影响因子：
6.1
通讯作者：
Wang, Zhouping
中科院分区：
文献类型：
--
作者：
Wu, Shijia;Wang, Yinqiu;Wang, Zhouping
建立了一种简单的检测副溶血性弧菌的比色适体传感器系统。磁性纳米粒子（MNP）是合成的，并与针对靶标的特异性适体缀合，用作捕获探针。此外，该方法采用金纳米颗粒（AuNPs）作为辣根过氧化物酶（HRP）和适体的载体，作为信号探针。在靶标存在的情况下，通过适配子与相应靶标的特异性识别，形成AuNPs-HRP-适配子-靶标-适配子-MNP的“双型”复合物。因此，限制在“夹心”复合物表面的HRP分子催化酶底物3，3 '，5，5'-四甲基联苯胺（TMB）和H2 O2并产生光信号。在最佳条件下，在对数图中，信号与10 - 10（6）个菌落形成单位（cfu）/mL的副溶血性弧菌浓度呈线性相关，检测限为10 cfu/mL。由于AuNPs，可以负载大量的HRP，导致信号放大，并且灵敏度将提高。这种策略有可能被扩展到构建简单的监测系统的各种生物分子与食品安全。
A simple colorimetric aptasensor system has been developed to detect Vibrio parahemolyticus. Magnetic nanoparticles (MNPs) are synthesized and conjugated with specific aptamers against target and used as capture probes. In addition, this method employs gold nanoparticles (AuNPs) as carriers of horseradish peroxidase (HRP) and aptamers, which served as signal probes. In the presence of target, a "sandwich-type" complex of AuNPs-HRP-aptamer-target-aptamer-MNPs is formed through specific recognition of aptamers and corresponding target. As a result, HRP molecules confined at the surface of the "sandwich" complexes catalyze the enzyme substrate, 3,3',5,5'-tetramethylbenzidine (TMB) and H2O2 and generate an optical signal. Under optimal conditions, the signals are linearly dependent on V. parahemolyticus concentrations from 10 to 10(6) colony-forming units (cfu)/mL in a logarithmic plot, with a limit of detection of 10 cfu/mL. Owing to AuNPs, a large amount of HRP could be loaded, resulting in an amplified signal, and the sensitivity would be improved. This strategy has the potential of being extended to the construction of simple monitor systems for a variety of biomolecules related to food safety.