Carbon dioxide can inhibit biofilms formation and cellular properties of Shewanella putrefaciens at both 30? and 4?
Carbon dioxide can inhibit biofilms formation and cellular properties of Shewanella putrefaciens at both 30? and 4?
复制标题
DOI：
10.1016/j.foodres.2022.111781
复制
发表时间：
2022-08-27
影响因子：
8.1
通讯作者：
Xie, Jing
中科院分区：
文献类型：
--
作者：
Li, Peiyun;Mei, Jun;Xie, Jing
腐败希瓦氏菌（Shewanella putrefaciens）（S.腐败菌（sputrefaciens）是一种常见的海洋鱼类特定腐败菌（SSO），即使在低温条件下也具有很强的腐败能力。CO2在水产品包装中被广泛应用于微生物控制。探讨CO2对S.腐殖菌生物膜的动态形成过程、细胞表面性质和细胞代谢特性。两个都是30度4？在纯CO2气体中进行了评价。通过结晶紫染色、激光共聚焦显微镜（CLSM）和场发射扫描电镜（FESEM）观察，揭示了S。腐殖菌生物膜被CO2明显延迟，细胞形态完整。S.腐败菌生物膜的浓度显著低于正常空气成分下的生物膜浓度。细胞表面性质的改变，如自聚集性和疏水性的降低，可能是CO2抑制生物膜的原因之一。CO2对细菌游动和群集运动能力的抑制作用也可以观察到，细菌的晕圈直径显著缩短。此外，通过ATP含量、ATP酶活性和细胞外蛋白水解活性的测定，发现CO2显著降低了细胞代谢。CO2的影响，无论是结合30？还是四个？而CO_2在培养温度为4 ℃时，抑制作用更明显。综上所述，S. CO2对腐败菌生物膜和细胞代谢特性有抑制作用。该研究为更好地应用CO2调控腐败微生物提供了理论依据。
Shewanella putrefaciens (S. putrefaciens), which is a common specific spoilage organism (SSO) of marine fish, has strong spoilage ability even under low-temperature conditions. Carbon dioxide (CO2) was widely applied to control microorganisms in aquatic products package. To explore the regulation mechanism of CO2 on biofilm formation and cell properties of S. putrefaciens, the dynamic formation process of biofilms, cellular surface properties, and cellular metabolic characteristics of S. putrefaciens at both 30 ? and 4 ? in pure CO2 gas were evaluated. As evidenced by the crystal violet staining method, confocal laser scanning microscopy (CLSM) analysis, and field emission scanning electron microscopy (FESEM) observation, dynamic formation process of S. putrefaciens biofilms was apparently delayed by CO2 with integral cellular morphology. The number and viability of sessile cells in S. putrefaciens biofilms was significantly lower than those in normal air composition. The changes in cellular surface properties, such as decreased auto-aggregation and hydrophobicity, might be one of the reasons why biofilms were inhibited by CO2. Inhibition of swimming and swarming motility ability by CO2 could also be observed with significantly shorter bacterial halo diameter. What's more, cellular metabolism was significantly decreased by CO2 according to the results of ATP content, ATPase activity and extracellular proteolytic activity. The influence of CO2 could be both observed whether combined with 30 ? or 4 ?. However, the inhibition produced by CO2 was more pronounced at the incubation temperature of 4 ?. In summary, it could be concluded that the dynamic formation process of S. putrefaciens biofilms and cellular metabolic prop-erties could be inhibited by CO2. This research provided a theoretical basis for better application of CO2 to regulate spoilage microorganisms.