Inherent antioxidant activity and high yield production of antioxidants in Phanerochaete chrysosporium
Inherent antioxidant activity and high yield production of antioxidants in Phanerochaete chrysosporium
复制标题
黄孢原平革菌固有的抗氧化活性和抗氧化剂的高产率
DOI：
10.1016/j.bej.2014.06.014
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发表时间：
2014-09-15
影响因子：
3.9
通讯作者：
He, Yibin
中科院分区：
文献类型：
--
作者：
Liu, Liang;Xu, Piao;He, Yibin
逆境环境中增加的活性氧物种(ROS)在环境应用过程中会对微生物造成氧化损伤。在这项研究中，我们强调了黄孢原毛霉作为抗氧化剂的一个新作用。黄孢原毛霉提取物具有显著的抗氧化活性，表现出剂量依赖的总抗氧化活性，并具有较强的清除O-2(中心点-)、中心点OH和H_2O_2的能力。此外，还发现黄孢原毛霉中存在多种抗氧化酶(过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶)和低分子抗氧化组分(谷胱甘肽、酚类和类黄酮类)。线性回归分析和皮尔逊相关系数分析证实，酶类和非酶类抗氧化剂参与了黄孢原毛霉的发酵过程，并作为“抗氧化剂网络”，与供试抗氧化剂之间的显著相关系数相吻合。此外，供试抗氧化剂对Cd暴露反应灵敏，其对SOD和酚类物质的刺激分别是2.78倍和2.35倍，这为抗氧化剂参与黄孢原毛平革菌的抗氧化防御系统提供了一个新的维度。本文提出了一种新的验证程序，利用黄孢原毛杆菌显著的抗氧化活性，具体验证其在环境治理中的良好耐受性和高效性。(C)2014爱思唯尔B.V.保留所有权利。
Incremental reactive oxygen species (ROS) under adversity environment could cause oxidative damages to microorganisms during environmental applications. In this study, we highlight a novel role of P. chrysosporium as antioxidants. The P. chrysosporium extracts possessed remarkable antioxidant activity, expressing a dose-dependent total antioxidant activity and accompanied with high reactive oxygen radical (O-2(center dot-), center dot OH and H2O2) scavenging capacity. In addition, it was established that, various compounds, such as antioxidant enzymes (catalase, superoxide dismutase and glutathione peroxidase) and low-molecular-weight antioxidant components (glutathione, phenolics and flavonoids) existed steadily in P. chrysosporium. Linear regression analysis and Pearson correlation coefficient analysis demonstrated that enzymatic and non-enzymatic antioxidants took part in processes and acted as "antioxidant network" in P. chrysosporium by confirming with the significant correlation coefficients among the tested antioxidants. Furthermore, those tested antioxidants were found sensitive response to Cd exposure, a 2.78-fold and 2.35-fold of stimulation has been found in SOD and phenolics, respectively, which provide a novel dimension to the involvement of antioxidants in the antioxidant defense system of P. chrysosporium. The paper proposes a new validation procedure to specifically validate the admirable tolerance and high efficiency of P. chrysosporium in environmental treatment application, taking advantage of the remarkable antioxidant activity. (C) 2014 Elsevier B.V. All rights reserved.