Prevention of postsurgery-induced abdominal adhesions by electrospun bioabsorbable nanofibrous poly(lactide-co-glycolide)-based membranes
Prevention of postsurgery-induced abdominal adhesions by electrospun bioabsorbable nanofibrous poly(lactide-co-glycolide)-based membranes
复制标题
DOI：
10.1097/01.sla.0000143302.48223.7e
复制
发表时间：
2004-11-01
影响因子：
9
通讯作者：
Chu, B
中科院分区：
文献类型：
--
作者：
Zong, XH;Li, S;Chu, B
目的：本研究的目的是评估非织造生物可吸收纳米纤维膜的聚（丙交酯-共-乙交酯）预防术后引起的腹部adhesions.Summary背景资料：最近的报告表明，目前用于粘连预防的材料只有有限的成功。其他生物可吸收材料使用新的制造技术的研究表明，产生一种改进的和廉价的产品，是适合于各种手术application.Methods：所有大鼠进行了中线剖腹手术的潜力。使用研磨垫对盲肠进行识别和评分，直至在前表面上观察到浆膜出血。直接在盲肠伤口上切除1 × 1 cm（2）的腹壁肌肉。然后在盲肠和腹壁之间放置屏障后立即将剖腹术闭合为2层（对照）。所有大鼠在28天后进行第二次剖腹术，以定性和定量地评估腹腔粘连的程度。盲肠粘连从对照组的78%减少到使用可生物降解聚乙烯的50%（丙交酯-共-乙交酯）（PLGA）非织造纳米纤维膜（n = 10，P = 0.2），而在使用含有PLGA和聚（乙二醇）/聚（D，L-丙交酯）（PEG-PLA）共混物的膜的组中为22%（n = 9，P = 0.03）。静电纺丝方法还使我们能够负载抗生素药物头孢西丁钠（Mefoxin; Merck Inc.，西点，PA），具有高功效。静电纺PLGA/PEG-PLA膜浸渍与5重量%头孢西丁钠，这相当于约10%的全身每日剂量通常采取手术后，在人类，完全防止盲肠粘连（0%）在rats.Conclusions：静电纺非织造生物可吸收纳米纤维膜的聚（丙交酯-共-乙交酯）是有效的，以减少粘连在损伤部位使用的目标大鼠模型。该膜充当物理屏障，但具有药物递送能力。组合物调节、载药能力和易于放置处理（相对疏水）的组合优点使得这些膜成为进一步临床评价的潜在成功候选物。
Objectives: The objective of this study was to evaluate the efficacy of nonwoven bioabsorbable nanofibrous membranes of poly(lactide-co-glycolide) for prevention of postsurgery-induced abdominal adhesions.Summary Background Data: Recent reports indicated that current materials used for adhesion prevention have only limited success. Studies on other bioabsorbable materials using a new fabrication technique demonstrated the promising potential of generating an improved and inexpensive product that is suitable for a variety of surgical applications.Methods: All rats underwent a midline celiotomy. The cecum was identified and scored using an abrasive pad until serosal bleeding was noted on the anterior surface. A 1 X 1 cm(2) of abdominal wall muscle was excised directly over the cecal wound. The celiotomy was then closed in 2 layers immediately (control) after a barrier was laid in between the cecum and the abdominal wall. All rats underwent a second celiotomy after 28 days to evaluate the extent of abdominal adhesions qualitatively and quantitatively.Results: Cecal adhesions were reduced from 78% in the control group to 50% in the group using biodegradable poly(lactide-co-glycolide) (PLGA) nonwoven nanofibrous membranes (n = 10, P = 0.2) and to 22% in the group using membranes containing PLGA and poly(ethylene glycol)/poly(D,L-lactide) (PEG-PLA) blends (n = 9, P = 0.03). Electrospinning method also enabled us to load an antibiotic drug Cefoxitin sodium (Mefoxin; Merck Inc., West Point, PA) with high efficacy. The electrospun PLGA/PEG-PLA membranes impregnated with 5 wt% cefoxitin sodium, which amounts to approximately 10% of the systemic daily dose typically taken after surgery in humans, completely prevented cecal adhesions (0%) in rats.Conclusions: Electrospun nonwoven bioabsorbable nanofibrous membranes of poly(lactide-co-glycolide) were effective to reduce adhesions at the site of injury using an objective rat model. The membrane acted as a physical barrier but with drug-delivery capability. The combined advantages of composition adjustment, drug-loading capability, and easy placement handling (relatively hydrophobic) make these membranes potentially successful candidates for further clinical evaluations.