Pharmaceutical analysis of 5-aminolevulinic acid in solution and in tissues
Pharmaceutical analysis of 5-aminolevulinic acid in solution and in tissues
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DOI：
10.1016/j.jphotobiol.2005.08.007
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发表时间：
2006-01-02
影响因子：
5.4
通讯作者：
Woolfson, AD
中科院分区：
文献类型：
--
作者：
Donnelly, RF;Morrow, DIJ;Woolfson, AD
5-氨基乙酰丙酸（ALA）溶液的定量以及用于实现这一点的方法已在文献中广泛报道。然而.经验证的方法很少被提出，也从未对这些方法进行过比较。由于在药物输送研究的必要性，优化和验证的方法来确定ALA在溶液中，本文比较，第一次，两个这样的方法验证国际协调会议（ICH）的标准。最重要的是，ALA与乙酰丙酮和甲醛的衍生化被认为是更适合于常规的荧光HPLC分析ALA比与邻苯二甲醛和2-巯基乙醇的衍生化。前一种方法成功地用于比较专有ALA乳膏和新型生物粘附贴剂系统的体外药物释放。此外，组织中ALA的测定对于比较不同的局部制剂（就其成功递送药物的能力而言）和不同的组织类型以评估其对药物渗透的屏障性质是必要的。因此，本文还描述了使用液体闪烁光谱作为一种分析工具，用于快速，方便和常规定量组织中的ALA，并确定局部应用乳膏和贴剂后的渗透深度。(C)2005 Elsevier B. V.保留所有权利。
Quantification of 5-aminolevulinic acid (ALA) ill solution, and methods used to achieve this, have been extensively reported in the literature. However. validated methods have only rarely been presented and never have methods been compared. Due to a necessity in drug delivery research for optimised and validated methods for determination of ALA in solution, this paper compares, for the first time, two such methods validated to International Conference on Harmonisation (ICH) standards. Of major importance, derivatisation of ALA with acetyl acetone and formaldehyde was found to be more suitable for routine fluorimetric HPLC analysis of ALA than derivatisation with o-phthaldialdehyde and 2-mercaptoethanol. This former method was successfully utilised in the comparison of in vitro drug release from a proprietary ALA cream and a novel bioadhesive patch-based system. In addition, determination of ALA in tissue is necessary to compare different topical formulations, in terms of their ability to deliver the drug successfully, and different tissue types, to assess their barrier properties to penetration of the drug. Consequently, this paper also describes the use of liquid scintillation spectroscopy as an analytical tool for rapid, convenient and routine quantification of ALA in tissue and determination of penetration depth to following topical application of creams and patches. (C) 2005 Elsevier B.V. All rights reserved.