Biochemical maturation of fetal rat lung: a comprehensive study including surfactant determination.
Biochemical maturation of fetal rat lung: a comprehensive study including surfactant determination.
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胎鼠肺的生化成熟：包括表面活性剂测定的综合研究。
DOI：
10.1159/000242684
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
C. Valenza
中科院分区：
文献类型：
--
作者：
J. Bourbon;P. Farrell;É. Doucet;D. Brown;C. Valenza
本文描述了一个全面的评估肺生长和生化成熟的胎儿和出生后早期大鼠。从孕16.5天到足月，胎肺生长快于全身生长。除了妊娠第19.5天至20.5天之间细胞生长减慢外，细胞数量呈准指数级增加。相比之下，在出生后的前两天，生长受到限制。干组织百分率和蛋白质含量在整个生育期内呈平行上升趋势。在整个肺组织磷脂酰胆碱的增加是最大的妊娠20.5和21.5天之间，而在孤立的表面活性成分，变化最显着的19.5和20.5天之间。最引人注目的变化是在妊娠第19.5天和产后1天之间的表面活性剂组分中磷脂酰胆碱增加了19倍，总磷脂浓度增加了12.5倍。二饱和磷脂酰胆碱（DSPC）浓度也发生了类似的变化。在同一时期，磷脂的残留物（非表面活性剂）馏分仅增加约2倍。这些数据表明，分离的表面活性剂组分的分析是有利的，在胎儿肺成熟的过程中，提供了一个非常敏感的指标，增加磷脂的生产。在雌性动物中检测到生化成熟的证据早于雄性动物，如19.5日龄雌性动物中表面活性剂组分DSPC浓度显著更大所示;然而，这种差异程度适中且非常短暂，因为在后期阶段不再明显。
The paper describes a comprehensive assessment of lung growth and biochemical maturation in the fetal and early postnatal rat. Fetal lung grew faster than whole body between gestational day 16.5 and term. Cell number increased quasi-exponentially, except for a slowing of cell growth between days 19.5 and 20.5 of gestation. By contrast, growth was limited during the two first postnatal days. Percentage of dry tissue and protein concentration increased in parallel during the whole period. Increases in whole lung tissue phosphatidylcholine were greatest between 20.5 and 21.5 days of gestation, whereas in isolated surfactant fraction, changes were most marked between 19.5 and 20.5 days. The most striking changes were a 19-fold increase in phosphatidylcholine and a 12.5-fold increase in the total phospholipid concentrations in the surfactant fraction between gestational day 19.5 and 1 day postpartum. Similar changes occurred in disaturated phosphatidylcholine (DSPC) concentration. During the same period, phospholipids of the residual (nonsurfactant) fraction increased only about 2-fold. These data indicate that analysis of an isolated surfactant fraction is advantageous in providing a very sensitive index of augmented phospholipid production during the process of fetal lung maturation. Evidence of biochemical maturation was detected earlier in females than in males, as indicated by a significantly larger surfactant fraction DSPC concentration in 19.5-day-old females; however, this difference was modest in degree and very transient, since it was no longer demonstrable in later stages.