ADULT T-CELL LEUKEMIA - ANTIGEN IN AN ATL CELL-LINE AND DETECTION OF ANTIBODIES TO THE ANTIGEN IN HUMAN-SERA
ADULT T-CELL LEUKEMIA - ANTIGEN IN AN ATL CELL-LINE AND DETECTION OF ANTIBODIES TO THE ANTIGEN IN HUMAN-SERA
复制标题
DOI：
10.1073/pnas.78.10.6476
复制
发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
MIYOSHI, I
中科院分区：
文献类型：
--
作者：
HINUMA, Y;NAGATA, K;MIYOSHI, I
某些人血清的间接免疫荧光显示，在日本西南部流行的成人T细胞白血病（ATL）患者的T细胞系MT-1的1-5%细胞的细胞质中存在抗原。在其他人淋巴细胞系中未检测到该抗原，包括6个T细胞系、7个B细胞系和4个非T非B细胞系。该抗原与Epstein-Barr病毒、单纯疱疹病毒、巨细胞病毒、水痘-带状疱疹病毒、松鼠猴疱疹病毒、马立克氏病病毒等疱疹病毒无交叉抗原性。携带抗原的细胞的比例增加了4倍。5-碘-2“-脱氧尿苷存在下培养。MT-1细胞中抗原的抗体被发现在所有44例ATL检查和40例恶性T细胞淋巴瘤（除了白血病细胞没有发现在外周血中的疾病，大多数与ATL相似）中的32。在ATL流行区的健康成人中也检测到26%的抗体，但在ATL非流行区的健康成人中仅检测到少数抗体。在EM上，在5-碘-2%-脱氧尿苷存在下培养的沉淀MT-1细胞中检测到细胞外C型病毒颗粒。
Indirect immunofluorescence of certain human sera demonstrated an antigen in the cytoplasm of 1-5% of the cells of a T-cell line, MT-1, from a patient with adult T-cell luekemia (ATL), which is endemic in southwestern Japan. The antigen was not detected in other human lymphoid cell lines including 6 T-cell lines, 7 B-cell lines and 4 non-T non-B cell lines. The antigen did not show cross antigenicity with those of herpesviruses including Epstein-Barr virus, herpes simplex virus, cytomegalovirus, varicella-zoster virus, herpesvirus saimiri and Marek disease virus. The proportion of antigen-bearing cells was increased by a factor of .apprxeq. 5 on culture in the presence of 5-iodo-2''-deoxyuridine. Antibodies against the antigen in MT-1 cells were found in all 44 patients with ATL examined and in 32 of 40 patients with malignant T-cell lymphomas (most had diseases similar to ATL except that leukemic cells were not found in the peripheral blood). The antibodies were also detected in 26% of the healthy adults examined from ATL-endemic areas but in only a few of those examined from ATL-non-endemic areas. On EM extracellular type C virus particles were detected in pelleted MT-1 cells cultured in the presence of 5-iodo-2%-deoxyuridine.