T lymphocyte cytotoxicity with natural varicella-zoster virus infection and after immunization with live attenuated varicella vaccine.
T lymphocyte cytotoxicity with natural varicella-zoster virus infection and after immunization with live attenuated varicella vaccine.
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DOI：
10.4049/jimmunol.142.2.636
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发表时间：
1989-01
影响因子：
4.4
通讯作者：
P. Diaz;S. Smith;E. Hunter;A. Arvin
中科院分区：
文献类型：
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作者：
P. Diaz;S. Smith;E. Hunter;A. Arvin
研究了水痘带状疱疹病毒（VZV）在急性原发性水痘病毒感染、自然免疫和接种减毒水痘活疫苗后的特异性细胞毒性，以水痘抗原（VZV Ag）和自体VZV超感染的淋巴母细胞样细胞系刺激PBMC产生的T细胞培养物（TCC）为目标。在急性感染受试者、自然免疫受试者和水痘疫苗接种者中，TCC观察到vzv感染的淋巴母细胞样细胞系的裂解。VZV糖蛋白I诱导细胞毒性T细胞，但杀伤效率低于VZV Ag刺激的TCC。TCC主要为CD4+ T淋巴细胞（平均86.6%），15.2%的细胞共表达il -19。TCC主要受HLA II类的限制，这表明使用I类单抗缺乏任何阻断作用，L243单抗对II类的阻断作用为15 - 71%。通过杀死K562细胞测量的无限制杀伤发生在所有TCC中，但与未感染的自体靶标相比，其杀伤作用最小。急性感染期间PBMC的表型表现为CD4+细胞的初始增加和循环中Leu-11+ (CD16)百分比的总体下降。与未感染的受试者相比，急性感染受试者的PBMC中没有K562杀伤增强。CD4+ CTL可能在急性水痘中起重要的原发性宿主应答作用。水痘减毒活疫苗免疫诱导vzv特异性记忆CTL反应，与自然免疫受试者相当。它们在原发VZV感染后持续很长时间，这可能表明CTL在VZV潜伏期再激活发作期间限制病毒复制的作用。
Varicella-zoster virus (VZV) specific cytotoxicity was investigated during acute primary VZV infection, in naturally immune subjects and after vaccination with the live attenuated varicella vaccine by using T cell cultures (TCC) generated by stimulating PBMC with VZV Ag and autologous VZV-superinfected lymphoblastoid cell lines as targets. Lysis of VZV-infected lymphoblastoid cell lines was observed by TCC from acutely infected subjects, naturally immune subjects, and recipients of the varicella vaccine. VZV glycoprotein I induced cytotoxic T cells but killing was less efficient than killing by TCC stimulated with VZV Ag. The TCC were primarily CD4+ (mean 86.6%) T lymphocytes with 15.2% of the cells coexpressing Leu-19. TCC were predominantly restricted by HLA class II as demonstrated by lack of any blocking using class I mAb and blocking of 15 to 71% by L243, a mAb to class II. Unrestricted killing as measured by killing of K562 cells occurred in all TCC but was minimally greater than that observed against uninfected autologous targets. Phenotypes of PBMC during acute infection had an initial increase in CD4+ cells and an overall decrease in the percentage of circulating Leu-11+ (CD16). No enhanced K562 killing was demonstrated in PBMC from subjects with acute infection compared to subjects without infection. CD4+ CTL may function as an important primary host response in acute varicella. Immunization with live attenuated varicella vaccine induced VZV-specific, memory CTL responses comparable to those of naturally immune subjects. The demonstration of their persistence long after primary VZV infection may indicate a role for CTL in restriction of viral replication during episodes of VZV reactivation from latency.