Modulation of immunocompetent cell populations by benzo[a]pyrene.
Modulation of immunocompetent cell populations by benzo[a]pyrene.
复制标题
通过苯并[a]芘调节免疫活性细胞群。
DOI：
10.1016/0041-008x(88)90126-3
复制
发表时间：
1988
影响因子：
3.8
通讯作者：
Bick,PH
中科院分区：
文献类型：
--
作者：
Blanton,RH;Myers,MJ;Bick,PH
这些研究检查了苯并[a]芘 (BaP) 诱导的免疫调节的细胞靶标。在体内暴露于 BaP 后评估小鼠单核白细胞的免疫能力。体外细胞分离和重构技术用于评估 T 依赖性抗体 (TDAb) 的产生并描述免疫调节事件。使用来自 BaP 和媒介物处理的小鼠的贴壁和非贴壁细胞群的组合重建培养物表明，这两个群体对 BaP 的免疫调节作用敏感。对贴壁细胞群的检查表明，少量暴露于 BaP 的辅助细胞增强了体外 TDAb 反应。这些反应大于含有暴露于媒介物的辅助细胞的类似培养物中观察到的反应。在含有正常贴壁细胞的培养物中对来自载体或 BaP 处理的小鼠分离的 B 细胞和 T 细胞进行重组，证明 B 细胞和 T 细胞对 BaP 的作用敏感。含有暴露于 BaP 的细胞群的培养物无法支持对照水平的抗体反应。结果表明，体内 BaP 暴露可通过对巨噬细胞、B 细胞和 T 细胞的影响来诱导免疫调节。
These studies examined the cellular targets of benzo[a]pyrene (BaP)-induced immunomodulation. The immunocompetence of murine mononuclear leukocytes was evaluated following in vivo exposure to BaP. In vitro cell separation and reconstitution techniques were used to evaluate T-dependent antibody (TDAb) production and delineate the immunomodulatory events. Reconstitution of cultures using combinations of adherent and nonadherent cell populations from BaP- and vehicle-treated mice demonstrated that both of these populations were sensitive to the immunomodulatory effects of BaP. Examination of the adherent cell population demonstrated that low numbers of BaP-exposed accessory cells enhanced in vitro TDAb responses. These responses were greater than those observed for analogous cultures containing vehicle-exposed accessory cells. Recombination of separated B cells and T cells from vehicle- or BaP-treated mice in cultures containing normal adherent cells demonstrated that both B cells and T cells were sensitive to the effects of BaP. Cultures containing BaP-exposed cell populations were incapable of supporting control level antibody responses. The results indicate that in vivo BaP exposure induces immunomodulation through effects on macrophages, B cells, and T cells.