Validation of an analytical method for the determination of spiramycin, virginiamycin and tylosin in feeding-stuffs by thin-layer chromatography and bio-autography
Validation of an analytical method for the determination of spiramycin, virginiamycin and tylosin in feeding-stuffs by thin-layer chromatography and bio-autography
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DOI：
10.1080/02652030601075470
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发表时间：
2007-03
期刊：
影响因子：
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通讯作者：
U. Vincent;G. Gizzi;C. Holst;J. D. Jong;J. Michard
中科院分区：
文献类型：
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作者：
U. Vincent;G. Gizzi;C. Holst;J. D. Jong;J. Michard
An inter-laboratory validation was carried out to determine the performance characteristics of an analytical method based on thin-layer chromatography (TLC) coupled to microbiological detection (bio-autography) for screening feed samples for the presence of spiramycin, tylosin and virginiamycin. Twenty-four samples including blank samples and samples with concentrations of the target analytes ranging between 1 and 5 mg kg−1 (expressed in microbiological activity) were analysed by seven laboratories participating in the study. The required detection limit was 1 mg kg−1 (expressed in microbiological activity). For spiramycin, acceptable values for the sensitivity (at least 95%) indicating the rate of correct positive results were obtained for samples containing this substance at or above 2 mg kg−1, whereas at 1 mg kg−1, the sensitivity rate dropped to about 70%. Therefore, it was concluded that the detection limit was 2 mg kg−1. For tylosin and virginiamycin, acceptable values of the sensitivity were obtained for all concentrations including 1 mg kg−1. Therefore, the method fulfils the criterion regarding the required sensitivity at the target detection limit for tylosin and virginiamycin.
