Target size of the ryanodine receptor from junctional terminal cisternae of sarcoplasmic reticulum.
Target size of the ryanodine receptor from junctional terminal cisternae of sarcoplasmic reticulum.
复制标题
肌浆网交界末端池的兰尼碱受体的目标大小。
DOI：
10.1021/bi00385a036
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Fleischer,S
中科院分区：
文献类型：
--
作者：
McGrew,SG;BoucekJr,RJ;McIntyre,JO;Jung,CY;Fleischer,S
1986年12月30日收到的修订稿摘要：对兰尼定受体进行靶向失活分析。该受体最近被认为是参与兴奋-收缩偶联的钙释放过程的通道，并定位于来自骨骼肌的肌浆网连接终池[Fleischer，S.，Ogan bunmi，E.M.，Dixon，M.C.，&Fleer，E.A.M.(1985)过程]。娜塔莉。阿卡德。SCI。美国[82,7256-7259]，照射交界区终池导致兰诺定结合随照射剂量呈指数下降，从而与靶点理论一致。目标分子量为138 000±21 000，即小于与兰诺定结合的多肽。以同一种膜制剂中的钙泵蛋白作为内对照，验证方法学。
Revised Manuscript Received December 30, 1986 abstract: Target inactivation analysis was carried out on the ryanodine receptor. This receptor recently has been implicated as the channel involved in the calcium releaseprocess in excitation-contraction coupling and was localized to the junctional terminal cisternae of sarcoplasmic reticulum from skeletal muscle [Fleischer, S., Ogunbunmi, E. M., Dixon, M. C., & Fleer, E. A. M.(1985) Proc. Natl. Acad. Sci. USA 82, 7256-7259], Irradiation of the junctional terminal cisternae resulted in an exponential decrease in ryanodine bindingwith radiation dose, thereby consistent with target theory. The target molecular weight was found to be 138 000±21 000, ie, smaller than the polypeptidethat binds ryanodine. The calcium pump protein in the same membrane preparation served as an internal control to validate the methodology.