GPCR Interacting Proteins-猫眼课题宝

GPCR 相互作用蛋白

DOI：

10.1007/978-1-59259-919-6_9

发表时间：

2005

期刊：

Nucleic Acids Research

影响因子：

14.9

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

Hongyan Wang;C. Willmore;Jia Bei Wang

配体刺激事件在G蛋白偶联受体（GPCR）的异三聚体G蛋白的复杂转导早已被赞赏。除此之外，最近的数据表明，其他蛋白质相互作用有助于并可以微调细胞信号。科学家们已经确定了其他直接或间接与GPCR相互作用的膜和细胞内蛋白质。事实上，在目前的文献中有50种或更多种蛋白质被描述为GPCR相互作用蛋白。GPCR相互作用蛋白作为配体诱发信号的调节剂。膜相关或细胞内GPCR相互作用蛋白在介导配体识别、信号转导优化、运输、受体聚集和/或区室化中具有关键作用。本章回顾了GPCR相互作用蛋白的四个方面的文献：（a）鉴定GPCR相互作用蛋白的方法;（B）GPCR上的相互作用结构域;（c）相互作用蛋白对GPCR信号事件的促进和微调;（d）μ阿片受体（μOR）相互作用蛋白的特殊分析。虽然GPCR二聚化被许多人视为GPCR之间的一种蛋白质相互作用，但与二聚体相关的蛋白质相互作用将不会被讨论;本书的另一部分致力于二聚化。

The complex transduction of ligand stimulation events at G-protein coupled receptors (GPCRs) by heterotrimetric G proteins has long been appreciated. In addition to this, recent data shows that other protein interactions assist and can fine-tune cellular signals. Scientists have identified other membrane and intracellular proteins that interact, directly or indirectly, with GPCRs. In fact, 50 or more proteins are described in current literature as GPCR interactive proteins. GPCR interacting proteins act as modulators of ligand-evoked signals. Membrane associated or intracellular GPCR interacting proteins have critical roles in mediating: ligand recognition, optimization of signal transduction, trafficking, receptor clustering, and/or compartmentalization. This chapter reviews four aspects of the GPCR interacting protein literature: (a) methods for identifying GPCR interacting proteins; (b) interaction domains on the GPCR; (c) facilitation and fine-tuning of GPCR signaling events by interacting proteins; and (d) particular analysis of proteins that are μ opioid receptor (μOR) interactive. Although GPCR dimerization is viewed by many as a type of protein interaction between the GPCRs, dimer-related protein interactions will not be discussed; an alternate section of this book is devoted to dimerization.