Chemoattraction of human T cells by IL-18
Chemoattraction of human T cells by IL-18
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DOI：
10.4049/jimmunol.170.2.1084
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发表时间：
2003-01-15
影响因子：
4.4
通讯作者：
Liew, FY
中科院分区：
文献类型：
--
作者：
Komai-Koma, M;Gracie, JA;Liew, FY
细胞运动对于诱导有效的免疫应答至关重要。我们在此报道了IL-1细胞因子家族成员IL-18对CD 4（+）T细胞的化学吸引作用。重组IL-18增加了体外极化形态的T细胞的比例，并以IL-18浓度梯度依赖的方式刺激其随后侵入胶原凝胶。免疫荧光显微镜研究确定对IL-18应答的主要细胞类型为IL-18（+）CD 4（+）。重要的是，类风湿性关节炎患者的滑膜CD 4（+）T细胞对IL-18有反应，采用极化形态和凝胶侵入，而没有进一步的体外激活，表明我们观察的生理相关性。最后，将rIL-18注射到DBA/1小鼠的足垫中导致炎性细胞的局部积聚。因此，这些数据首次证明了IL-1细胞因子家族成员的淋巴细胞化学引诱物特性及其在炎性疾病中的相关性。
Cell locomotion is crucial to the induction of an effective immune response. We report here the chemoattraction of CD4(+) T cells by IL-18, a member of the IL-1 cytokine family. Recombinant IL-18 increased the proportion of T cells in polarized morphology in vitro and stimulated their subsequent invasion into collagen gels in an IL-18 concentration gradient-dependent manner. Immunofluorescent microscopy studies determined that the major cell type responding to IL-18 was IL-18(+)CD4(+). Importantly, synovial CD4(+) T cells from patients with rheumatoid arthritis responded to IL-18, adopting polarized morphology and gel invasion without further activation ex vivo, indicating the physiologic relevance of our observations. Finally, injection of rIL-18 into the footpad of DBA/1 mice led to local accumulation of inflammatory cells. These data therefore demonstrate for the first time lymphocyte chemoattractant properties of a member of the IL-1 cytokine family and its relevance in inflammatory diseases.