CHARACTERIZATION OF A NEW IL-6-DEPENDENT HUMAN B-LYMPHOMA CELL-LINE IN LONG-TERM CULTURE
CHARACTERIZATION OF A NEW IL-6-DEPENDENT HUMAN B-LYMPHOMA CELL-LINE IN LONG-TERM CULTURE
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DOI：
10.1016/1043-4666(93)90039-8
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发表时间：
1993-09-01
期刊：
影响因子：
3.8
通讯作者：
NELSON, DL
中科院分区：
文献类型：
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作者：
BOCK, GH;LONG, CA;NELSON, DL
我们在长期培养中建立了b细胞系DS-1细胞系。它来源于免疫缺陷的肠淋巴管扩张和淋巴瘤患者，通过体外培养恶性胸腔积液细胞与IL-6。细胞表面表型为：PCA-1， HLA II类（+）；Cd25, cd19, cd20, cd30, cd38（−）细胞在培养基中的增殖能力较差，并且在人源的il -6而不是小鼠源的il -6的反应中表现出8倍的剂量依赖性增殖。il -6对DS-1向培养上清分泌IgG没有促进作用。虽然用小鼠B9杂交瘤细胞进行生物测定或ELISA均未检测到组成性IL-6的产生，但通过Northern分析，在polyA+选择的mRNA中检测到IL-6信息的存在。中和IL-6多克隆抗体（37%）、IL-6单抗（54%）和IL-6R单抗（53%）可抑制DS-1细胞的自发增殖。通过放射性标记结合和Scatchard分析，DS-1表达高亲和力和低亲和力IL-6受体（分别为Kd1.2 × 10−11和6.7 × 10−10）。因此，DS-1代表了一种自分泌il -6的b细胞系，类似于艾滋病和其他免疫缺陷疾病患者中出现的淋巴样恶性肿瘤。尽管构成性IL-6产生，但DS-1的体外生长依赖于外源IL-6。因此，DS-1可能作为IL-6依赖性模型有用。该细胞系也可能促进免疫功能低下患者b细胞淋巴瘤诊断和治疗策略的发展。
We have established a cell line (DS-1) of B-cell lineage in long-term culture. It was derived from an immunodeficient patient with intestinal lymphangiectasia and lymphoma by culturing malignant pleural effusion cells with IL-6 in vitro. The cell surface phenotype was: PCA-1, HLA Class II(+); CD25, CD19, CD20, CD30, CD38(−). Cell proliferation was poor in medium and exhibited an eight-fold, dose-dependent increase of proliferation in response to rIL-6 of human but not murine origin. The secretion of IgG into culture supernatants by DS-1 was not enhanced by rIL-6. While constitutive production of IL-6 was not detected by bioassay using murine B9 hybridoma cells or by ELISA, the presence of IL-6 message was detected in polyA+ selected mRNA by Northern analysis. Spontaneous proliferation of DS-1 cells was inhibited by neutralizing polyclonal antibodies to IL-6 (37%) and mAb to IL-6 (54%) and IL-6R (53%). DS-1 expressed both high and low affinity IL-6 receptors (Kd1.2 × 10−11and 6.7 × 10−10, respectively) by radiolabelled binding and Scatchard analysis. Thus, DS-1 represents an autocrine IL-6-producing cell line of B-cell lineage which resembles lymphoid malignancies arising in patients with AIDS and other immunodeficiency diseases. Despite constitutive IL-6 production, the in vitro growth of DS-1 is dependent upon exogenous IL-6. DS-1 may thus be useful as a model of IL-6 dependency. This cell line may also facilitate development of strategies for diagnosis and treatment of B-cell lymphomas in immunocompromised patients.