Cloning and characterization of IL-1HY2, a novel interleukin-1 family member
Cloning and characterization of IL-1HY2, a novel interleukin-1 family member
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DOI：
10.1074/jbc.m010095200
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发表时间：
2001-06-08
影响因子：
4.8
通讯作者：
Ford, JE
中科院分区：
文献类型：
--
作者：
Lin, HS;Ho, AS;Ford, JE
白细胞介素 - 1（IL - 1）家族成员在炎症和宿主防御过程中发挥着重要作用。在此我们描述了IL - 1家族一个新成员IL - 1HY2的鉴定和特征。人IL - 1HY2蛋白与IL - 1受体拮抗剂具有显著的氨基酸序列相似性（37%），并且其预测的三维结构与IL - 1受体拮抗剂相似。IL - 1HY2基因位于人类2号染色体上靠近其他IL - 1家族基因的位置，并且IL - 1HY2基因的基因组结构与其他IL - 1家族成员高度保守。IL - 1HY2蛋白由哺乳动物细胞分泌，纯化的重组IL - 1HY2蛋白可结合可溶性I型IL - 1受体。IL - 1HY2在人皮肤、脾脏和扁桃体中表达。免疫组织化学分析表明，IL - 1HY2蛋白在皮肤基底上皮细胞和扁桃体中增殖的B细胞中表达。这些数据表明IL - 1HY2是一种新的IL - 1家族成员，它可能参与IL - 1家族成员网络以调节适应性和先天性免疫应答。
The interleukin-1 (IL-1) family members play an important role in the process of inflammation and host defense. We describe here the identification and characterization of a novel member of the IL-1 family, IL-1HY2, The human IL-1HY2 protein shares significant amino acid sequence similarity (37%) with the IL-1 receptor antagonist and has a predicted three-dimensional structure similar to that of the IL-1 receptor antagonist. The IL-1HY2 gene is located in close proximity to other IL-1 family genes on human chromosome 2, and the genomic organization of the IL-1HY2 gene is highly conserved with other IL-1 family members. IL-1HY2 protein is secreted from mammalian cells, and the purified recombinant IL-1HY2 protein binds soluble IL-1 receptor type I. IL-1HY2 is expressed in human skin, spleen, and tonsil. Immunohistochemical analysis showed that the IL-1HY2 protein is expressed in the basal epithelia of skin and id proliferating B cells of the tonsil. These data suggest that IL-1HY2 is a novel IL-1 family member and that it may participate in a network of IL-1 family members to regulate adapted and innate immune responses.