MICROTUBULE FORMATION AND NEURITE GROWTH IN CEREBELLAR MACRONEURONS WHICH DEVELOP INVITRO - EVIDENCE FOR THE INVOLVEMENT OF THE MICROTUBULE-ASSOCIATED PROTEINS, MAP-1A, HMW-MAP2 AND TAU
MICROTUBULE FORMATION AND NEURITE GROWTH IN CEREBELLAR MACRONEURONS WHICH DEVELOP INVITRO - EVIDENCE FOR THE INVOLVEMENT OF THE MICROTUBULE-ASSOCIATED PROTEINS, MAP-1A, HMW-MAP2 AND TAU
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DOI：
10.1016/0165-3806(89)90023-0
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发表时间：
1989-10-01
期刊：
影响因子：
--
通讯作者：
CACERES, A
中科院分区：
文献类型：
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作者：
FERREIRA, A;BUSCIGLIO, J;CACERES, A
在培养的胚胎小脑宏神经元中研究了微管相关蛋白（MAP）的表达与微管形成之间的关系。获得的结果表明，在这些神经元中，高分子量 MAP2 (HMW-MAP2) 充当微管蛋白组装的启动子，因为它的诱导和分布模式与微管质量的增加高度相关，微管质量的增加与轴突和树突的生长平行。 MAP-1a 可能具有类似的作用，但仅限于树突微管的组装。另一方面，Tau 的表达和积累遵循与稳定微管诱导相同的时间过程；此外，在神经突分化和生长的所有阶段，与其他 MAP 相比，该蛋白似乎优先与该微管子集相关，观察结果表明该蛋白在决定轴突和树突伸长过程中微管稳定性方面发挥着重要作用。 最后，目前的结果表明环境刺激能够调节这些 MAP 的表达；它们各自的感应随着信号类型的变化而变化。因此，虽然可扩散物质能够显着诱导 HMW-MAP2，但 MAP-1a 和 Tau 的诱导取决于细胞基质附着和/或细胞间相互作用。
The relationship between the expression of microtubule-associated proteins (MAPs) and microtubule formation was studied in embryonic cerebellar macroneurons maintained in culture. The results obtained suggest that in these neurons high molecular weight-MAP2 (HMW-MAP2) acts as a promoter of tubulin assembly since its induction and pattern of distribution are highly correlated with the increase in microtubule mass which parallels axonal and dendritic growth; MAP-1a may have a similar role but restricted to the assembly of dendritic microtubules. On the other hand, Tau expression and accumulation follows a time course identical to that of the induction of stable microtubules; besides, at all stages of neurite differentiation and growth this protein seems to be preferentially associated with this subset of microtubules as opposed to the other MAPs, observations which suggest an important role for this protein in determining microtubule stability during axonal and dendritic elongation. Finally, the present results show that environmental stimuli are capable of regulating the expression of these MAPs; the induction of each of them varies as a function of the type of signal. Thus, while diffusable substances are able to dramatically induce HMW-MAP2, MAP-1a and Tau inductions depend on cell substrate attachment and/or cell-cell interactions.