NUCLEAR LAMINS AND PERIPHERAL NUCLEAR ANTIGENS DURING FERTILIZATION AND EMBRYOGENESIS IN MICE AND SEA-URCHINS
NUCLEAR LAMINS AND PERIPHERAL NUCLEAR ANTIGENS DURING FERTILIZATION AND EMBRYOGENESIS IN MICE AND SEA-URCHINS
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DOI：
10.1073/pnas.82.14.4727
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
BROWN, DL
中科院分区：
文献类型：
--
作者：
SCHATTEN, G;MAUL, GG;BROWN, DL
在小鼠受精和胚胎发育过程中，以及海胆的核结构变化之后，用抗核层蛋白A/C和B的抗体以及针对核和染色体外围的抗原的抗体进行观察。在小鼠受精过程中，在所有的原核和细胞核上都发现了板层蛋白，但在第二极体核上的强度有所减弱。在这两个系统的精子上，层蛋白减少，仅在顶体和中心粒窝处检测到。在海胆卵中，在两个原核上都发现了板蛋白。与其他分裂细胞不同，海胆卵和胚胎的有丝分裂染色体与板层保持着联系。外周抗体描绘了除成熟的小鼠精子核以外的每条染色体和细胞核。在早期发育过程中，板层蛋白的分布发生了与预期的巨大变化。在小鼠桑椹胚或囊胚中，尽管层蛋白B仍然存在，但层蛋白A/C不再被识别。在海胆中，用多克隆抗体检测到的层蛋白A/C和层蛋白B在囊胚期后都会丢失，尽管出现了不同的层蛋白A/C表位，被单抗识别。在这两个系统中，原核的形成都涉及到新的联结或板层的暴露。极体核在很大程度上被限制在片层的细胞质池中。快速增殖的海胆卵中的有丝分裂染色体保留了相关的片层。不同板层的表达或暴露的变化是胚胎发生的一个共同特征。
Nuclear structural changes during fertilization and embryogenesis in mice and in sea urchins were followed by using antibodies against the nuclear lamins A/C and B and against antigens at the periphery of nuclei and chromosomes. Lamins are found on all pronuclei and nuclei during mouse fertilization, but with a diminished intensity on the 2nd polar body nucleus. On sperm in both systems, lamins are reduced and detected only at the acrosomal and centriolar fossae. In sea urchin eggs, lamins are found on both pronuclei. Unlike in other dividing cells, the mitotic chromosomes of sea urchin eggs and embryos retain an association with lamins. The peripheral antibodies delineate each chromosome and nucleus except the mature mouse sperm nucleus. A dramatic change from the expected lamin distribution occurs during early development. In mouse morulae or blastocysts, lamins A/C are no longer recognized, although lamin B remains. In sea urchins both lamins A/C and lamin B, as detected with polyclonal antibodies, are lost after the blastula stage, although a different lamin A/C epitope emerges as recognized by a monoclonal antibody. Pronucleus formation in both systems involves a new association or exposure of lamins. The polar body nucleus is largely restricted from the cytoplasmic pool of lamins. Mitotic chromosomes in the rapidly proliferating sea urchin egg retain associated lamins. Changes in the expression or exposure of different lamins are a common feature of embryogenesis.