ESCHERICHIA COLI CITRATE SYNTHASE . PURIFICATION AND EFFECT OF POTASSIUM ON SOME PROPERTIES
ESCHERICHIA COLI CITRATE SYNTHASE . PURIFICATION AND EFFECT OF POTASSIUM ON SOME PROPERTIES
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DOI：
10.1021/bi00839a041
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发表时间：
1969-01-01
期刊：
影响因子：
2.9
通讯作者：
SRERE, PA
中科院分区：
文献类型：
--
作者：
FALOONA, GR;SRERE, PA
结果纯化。冷冻E.大肠杆菌冻干并在-40 ℃下保存。除非另有说明，否则所有步骤均在0 ° C下进行。声波治疗在Waring Blendor中，将冻干的细胞（130 g）快速分散在5体积的0.1M磷酸钾缓冲液（pH 7.0）中。将悬浮的细胞置于冰浴包围的烧杯中，并在恒定搅拌下，通过超声处理用设定为最大输出的Bronwill Biosonik破碎。几滴
ResultsPurification. Frozen E. coli were lyophilized and stored at—40. All steps were carried out at 0^ 1 unless otherwise specified.Step 1. Sonic treatment. Lyophilized cells (130 g) were rapidly dispersed in five volumes of 0.1 m potassium phosphate buffer (pH 7.0) in a Waring Blendor. The suspended cells were placed in a beaker surrounded by an ice bath and with constant stirring were disrupted by sonication with a Bronwill Biosonik set at maximum output. A few drops of