Response of genes associated with mitochondrial function to mild heat stress in yeast Saccharomyces cerevisiae
Response of genes associated with mitochondrial function to mild heat stress in yeast Saccharomyces cerevisiae
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DOI：
10.1093/jb/mvg155
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发表时间：
2003-09-01
影响因子：
2.7
通讯作者：
Mihara, K
中科院分区：
文献类型：
--
作者：
Sakaki, K;Tashiro, K;Mihara, K
芽殖酵母的全基因组表达模式。使用 DNA 微阵列分析了酿酒酵母对不可发酵碳源中温和热处理的反应。在检查的 5,870 个开放阅读框（核基因组转录本）中，当细胞从 25 摄氏度转变为 37 摄氏度时，104 个基因上调，287 个基因下调。 40 个上调基因和 235 个下调基因编码定位指定蛋白。在113个热抑制基因（不包括122个热抑制核糖体基因）中，36个是线粒体相关基因，而40个热诱导基因中只有2个与线粒体相关。特别是，涉及线粒体呼吸链的 9 个基因和涉及线粒体蛋白质易位的 7 个基因被显着抑制，表明线粒体呼吸功能和生物合成被下调。与这些发现一致的是，不可发酵碳源中酵母细胞的生长在 37°C 下受到抑制，并且与来自未应激细胞的线粒体相比，从热应激细胞中分离的线粒体表现出受损的前蛋白输入活性。相比之下，许多参与糖酵解和通过三羧酸循环产生谷氨酸的代谢途径的基因被上调，而三羧酸循环对于生物合成反应至关重要。酵母细胞可能下调线粒体功能以规避热诱导的氧化应激，上调应激相关基因，并重塑代谢途径基因以应对轻度热应激：这是一种以细胞生长为代价的适应性反应。
The genome-wide expression pattern of budding yeast Saccharomyces. cerevisiae in response to mild heat treatment in a non-fermentable carbon source was analyzed using DNA microarrays. Of 5,870 open reading frames (nuclear genome transcripts) examined, 104 genes were upregulated and 287 genes were downregulated upon shifting of the cells from 25degreesC to 37degreesC. Forty upregulated genes and 235 downregulated genes encoded localization-assigned proteins. Of 113 heat-repressible genes (excluding 122 heat-repressible ribosomal genes), 36 were mitochondria-related genes, whereas only 2 of 40 heat-inducible genes were mitochondria-related. In particular, 9 genes involved in the mitochondrial respiratory chain and 7 genes involved in mitochondrial protein translocation were significantly repressed, suggesting that mitochondrial respiratory function and biogenesis were downregulated. Consistent with these findings, the growth of yeast cells in a non-fermentable carbon source was repressed at 37degreesC and the mitochondria isolated from heat-stressed cells exhibited compromised preprotein-import activity compared with those from unstressed cells. In contrast, many genes involved in glycolysis and the metabolic pathway to produce glutamate via the tricarboxylic acid cycle, which is essential for biosynthetic reactions, were upregulated. Yeast cells might downregulate mitochondrial function to circumvent heat-induced oxidative stress, upregulate stress-related genes, and remodel genes for metabolic pathways in response to mild heat stress: an adaptive response at the expense of cell growth.