Susceptibility of Aedes flavopictus miyarai and Aedes galloisi mosquito species in Japan to dengue type 2 virus
Susceptibility of Aedes flavopictus miyarai and Aedes galloisi mosquito species in Japan to dengue type 2 virus
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DOI：
10.1016/j.apjtb.2016.03.003
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发表时间：
2016-05-01
影响因子：
1.7
通讯作者：
Eshita, Yuki
中科院分区：
文献类型：
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作者：
Srisawat, Raweewan;Phanitchat, Thipruethai;Eshita, Yuki
目的：方法：采用血清型2 ThNH 28/93测定日本黄纹伊蚊（Ae. F. miyarai）、白纹伊蚊Aedes galloisi（Ae. galloisi）和白纹伊蚊（Ae. albopictus），在日本收集。我们使用来自泰国的埃及伊蚊作为阳性对照。这些蚊子通过胸腔内或口腔感染病毒。在感染后10或14天，解剖蚊子，并从它们的触角、胸部、头部和腿部提取总RNA。用登革病毒特异性引物进行RT-PCR检测，以评价苔藓对登革病毒的敏感性。不同蚊种的感染率和死亡率的差异进行了测试，使用Fisher的精确概率test.Results：登革病毒的感染率给Ae。F.宫井山在感染后第10天，通过RT-PCR检测，galloisi和埃及伊蚊是相同的。我们检测的所有身体部位都对登革热病毒呈RT-PCR阳性。对于口服病毒，AE不同身体部位的感染率。F.宫井蚊的种群数量略高于Ae.白纹伊蚊的感染率与对照组差异无显著性（P>0.05）。死亡率为Ae。F. miyarai和Ae.白纹伊蚊与白纹伊蚊相似（P = 0.19）。我们的数据表明，登革病毒能够复制和传播到所有四种蚊子（日本和泰国）的继发感染部位。在日本，白纹伊蚊是登革热传播的已知候选者。然而，我们的数据表明，Ae。F.来自石垣岛（靠近冲绳）的miyarai和Ae.来自北海道（北方日本）的鸡长角体病也应该被视为这些地区登革热传播的潜在媒介。应进一步研究这些蚊子。
Objective: To evaluate the potential of local mosquitoes to act as vectors for dengue transmission in Japan.Methods: Serotype 2ThNH28/93 was used to test the dengue susceptibility profiles of Aedes flavopictus miyarai (Ae. f. miyarai), Aedes galloisi (Ae. galloisi) and Aedes albopictus (Ae. albopictus), which were collected in Japan. We used Aedes aegypti from Thailand as a positive control. The mosquitoes were infected with the virus intrathoracically or orally. At 10 or 14 days post infection, the mosquitoes were dissected and total RNA was extracted from their abdomens, thoraxes, heads and legs. Mosquito susceptibility to dengue virus was evaluated using RT-PCR with dengue virus-specific primers. Differences in the infection and mortality rates of the different mosquito species were tested using Fisher's exact probability test.Results: The infection rates for dengue virus administered intrathoracically to Ae. f. miyarai, Ae. galloisi and Aedes aegypti mosquitoes were identical by RT-PCR on Day 10 post infection. All of the body parts we tested were RT-PCR-positive for dengue virus. For the orally administered virus, the infection rates in the different body parts of the Ae. f. miyarai mosquitoes were slightly higher than those of Ae. albopictus mosquitoes, but were similar to the control mosquitoes (P>0.05). The mortality rates for Ae. f. miyarai and Ae. albopictus mosquitoes were similar (P = 0.19). Our data indicated that dengue virus was able to replicate and disseminate to secondary infection sites in all of the four mosquito species (Japanese and Thai).Conclusions: Ae. albopictus is a well-known candidate for dengue transmission in Japan. However, our data suggest that Ae. f. miyarai from Ishigaki Island (near Okinawa Island) and Ae. galloisi from Hokkaido (Northern Japan) should also be regarded as potential vectors for dengue transmission in these regions. Further studies on these mosquitoes should be conducted.