Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions.
Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions.
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DOI：
10.7554/elife.28415
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发表时间：
2017-12-04
期刊：
影响因子：
7.7
通讯作者：
Kulesa PM
中科院分区：
文献类型：
--
作者：
Morrison JA;McLennan R;Wolfe LA;Gogol MM;Meier S;McKinney MC;Teddy JM;Holmes L;Semerad CL;Box AC;Li H;Hall KE;Perera AG;Kulesa PM
神经嵴细胞在胚胎中迁移，但细胞如何以定向和集体的方式移动仍不清楚。在此，我们对鸡的颅神经嵴细胞在三个连续阶段的迁移进行了首次单细胞转录组分析，并确定和建立了细胞位置与时间特异性转录特征之间的层级关系。我们确定了最具侵袭性的神经嵴“开拓者”细胞的一种新的转录特征，该特征在迁移过程中保持一致，并富集了约900个基因。对几个“开拓者”基因的敲低显示迁移的总距离有显著但适度的变化。然而，通过RNAscope和免疫组织化学进行的体内表达分析揭示了一些散在分布的模式，包括在迁移流的其他子区域内的细胞中强烈的单个“开拓者”基因表达。这些数据为神经嵴细胞迁移流内的分子多样性和动态提供了新的见解，而这些分子多样性和动态是复杂的定向和集体细胞行为的基础。
Neural crest cells migrate throughout the embryo, but how cells move in a directed and collective manner has remained unclear. Here, we perform the first single-cell transcriptome analysis of cranial neural crest cell migration at three progressive stages in chick and identify and establish hierarchical relationships between cell position and time-specific transcriptional signatures. We determine a novel transcriptional signature of the most invasive neural crest Trailblazer cells that is consistent during migration and enriched for approximately 900 genes. Knockdown of several Trailblazer genes shows significant but modest changes to total distance migrated. However, in vivo expression analysis by RNAscope and immunohistochemistry reveals some salt and pepper patterns that include strong individual Trailblazer gene expression in cells within other subregions of the migratory stream. These data provide new insights into the molecular diversity and dynamics within a neural crest cell migratory stream that underlie complex directed and collective cell behaviors.