A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome.
A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome.
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用于识别 O157:H7 肠出血性大肠杆菌和导致出血性结肠炎和溶血性尿毒症综合征的其他血清型的 DNA 探针。
DOI：
10.1093/infdis/156.1.175
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Wachsmuth,K
中科院分区：
文献类型：
--
作者：
Levine,MM;Xu,JG;Kaper,JB;Lior,H;Prado,V;Tall,B;Nataro,J;Karch,H;Wachsmuth,K
肠出血性大肠杆菌可引起出血性结肠炎和溶血性尿毒症综合征，产生有效的细胞毒素(VT毒素或志贺样毒素)，并拥有一个编码新菌毛抗原并促进与上皮细胞黏附的质粒(∼60兆)。该探针与107株O157：H7中的106株(99%)和44株O26：H11中34株(77%)的VT阳性菌株杂交，并与26株VT阳性E中的21株(81%)杂交。出血性结肠炎和HUS患者的O157：H7或O26：H11以外的血清型。我们检测了601株其他菌株，其中包括18株H11以外的H型O26菌株，306株肠致病性E。大肠埃希菌60株，肠侵袭性E。大肠埃希菌119株，肠毒素E。大肠埃希菌，尿路分离株20株，正常肠道菌群分离株77株，仅1株(O127：H-)阳性(特异性99.8%)。血清型O26：H11，以前被认为是一种典型的肠道病原学E。大肠埃希氏菌，现在被证明是EHEC。
EnterohemorrhagicEscherichia coli(EHEC) cause hemorrhagic colitis and hemolytic uremic syndrome (HUS), make potent cytotoxins (Verotoxins [VT] or Shiga-like toxins), and possess a plasmid (∼60 megadaltons) that encodes a new fimbrial antigen and promotes attachment to epithelial cells.Weevaluated the use of a DNA probe, prepared from a 3.4-kilobase segment of the EHEC plasmid, to detect EHEC. The probe hybridized with 106 (99%) of 107 O157:H7 and 34 (77%) of 44 O26:H11, VT-positive strains from patients with colitis, HUS, and diarrheal disease and hybridized with 21 (81%) of 26 VT-positiveE. coliof serotypes other than O157:H7 or O26:H11 from patients with hemorrhagic colitis and HUS. Weexamined 601 other strains, including 18 serotype O26 isolates of H types other than H11, 306 enteropathogenicE. coli, 60 enteroinvasiveE. coli, 119 enterotoxigenicE. coli, and 20 isolates from the urinary tract and 77 isolates from the normal intestinal flora; only one (O127:H-) was positive (specificity, 99.8%). Serotype O26:H11, previously considered a classic enteropathogenicE. coliserotype, is now shown to be EHEC.