The role of depletion of dimethyl sulfoxide before autografting:: On hematologic recovery, side effects, and toxicity
The role of depletion of dimethyl sulfoxide before autografting:: On hematologic recovery, side effects, and toxicity
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DOI：
10.1016/j.bbmt.2003.09.016
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发表时间：
2004-02-01
影响因子：
4.3
通讯作者：
Glück, S
中科院分区：
文献类型：
--
作者：
Syme, R;Bewick, M;Glück, S
从外周血或骨髓采集干细胞用于自体移植后，需要用二甲基亚砜（DMSO）进行保护。不幸的是，当解冻的细胞悬液中含有DMSO并输注时，可能会引发严重的并发症和副作用。为了评估在自体移植前去除DMSO是否会影响安全性和有效性，56名同意接受大剂量化疗和自体血干细胞移植的连续患者被分配接受未经处理的或去除DMSO的自体移植物。在移植当天，冷冻保存的细胞被解冻，并立即或在添加了6%抗凝枸橼酸葡萄糖溶液的生理盐水中洗涤3次后输注给患者。在解冻前后以及洗涤后进行细胞计数及活力检测、集落形成试验和表型分析。记录血液学恢复情况、副作用和并发症。对56名患者的体外和临床数据显示，在自体移植前体外去除DMSO不会导致细胞数量、活力、粒细胞 - 巨噬细胞集落形成单位活性或CD34(+)细胞数量的显著损失。此外，它还能实现安全且持续的植入。通过持续监测记录到的并发症和副作用明显减少；然而，该过程每个患者需要3到4小时的实验室工作。（C）2004年美国血液和骨髓移植学会。
Cryopreservation of stem cells after collection from peripheral blood or bone marrow for autologous transplantation necessitates protection with dimethyl sulfoxide (DMSO). Unfortunately, DMSO, when infused with the thawed cell suspension, may induce serious complications and side effects. To assess whether depletion of DMSO before autografting affects safety and efficacy, 56 consenting consecutive patients treated with high-dose chemotherapy and autologous blood stem cell transplantation were assigned to obtain either an untreated or DMSO-depleted autograft. On the day of transplantation, the cryopreserved cells were thawed and infused to the patient either immediately or after washing 3 times in normal saline supplemented with 6% anticoagulant citrate dextrose solution. Cell count with viability, clonogenic assay, and phenotyping were performed before and after thawing and after washing. Hematologic recovery, side effects, and complications were recorded. The in vitro and clinical data on 56 patients show that the depletion of DMSO in vitro before autografting does not induce a significant loss of cell number, viability, colony-forming unit- granulocyte-macrophage activity, or number of CD34(+) cells. Furthermore, it leads to a safe and sustained engraftment. The complications and side effects, as recorded by continuous monitoring, were substantially less; however, the procedure takes 3 to 4 hours of laboratory work per patient. (C) 2004 American Society for Blood and Marrow Transplantation.