Region-Specific and Pregnancy-Enhanced Vasodilator Effects of Hydrogen Sulfide.
Region-Specific and Pregnancy-Enhanced Vasodilator Effects of Hydrogen Sulfide.
复制标题
DOI：
10.26502/ogr0145
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
硫化氢（H2S）是一种引起血管平滑肌细胞血管舒张的心血管信号分子，但其机制尚不清楚。我们研究了H2S如何影响非妊娠和妊娠大鼠肠系膜和子宫动脉的内皮细胞和潜在的机制。H2S供体GYY 4137和NaHS在妊娠和非妊娠大鼠中舒张子宫动脉大于肠系膜动脉。GYY 4137和NaHS引起更大的松弛，在子宫动脉的怀孕与非妊娠大鼠。高细胞外K+废除NaHS松弛在怀孕的子宫动脉，表明钾通道参与。NaHS松弛不受电压门控钾通道阻滞剂的影响，ATP敏感性钾通道阻滞剂减少，钙激活钾（BKCa）通道阻滞剂消除。巯基还原剂二硫苏糖醇也阻止了NaHS的松弛。因此，H2S在子宫动脉中具有区域特异性和妊娠增强的血管舒张作用，主要由BKCa通道和硫水合作用介导。
Hydrogen sulfide (H2S) is a cardiovascular signaling molecule that causes vasodilation in vascular smooth muscle cells, but its mechanism is unclear. We examined how H2S affects mesenteric and uterine arteries without endothelium in nonpregnant and pregnant rats and the underlying mechanisms. H2S donors GYY4137 and NaHS relaxed uterine arteries more than mesenteric arteries in both pregnant and nonpregnant rats. GYY4137 and NaHS caused greater relaxation in the uterine artery of pregnant versus nonpregnant rats. High extracellular K+ abolished NaHS relaxation in pregnant uterine arteries, indicating potassium channel involvement. NaHS relaxation was unaffected by voltage-gated potassium channel blockers, reduced by ATP-sensitive potassium channel blockers, and abolished by calcium-activated potassium (BKCa) channel blockers. Thiol-reductant dithiothreitol also prevented NaHS relaxation. Thus, H2S has region-specific and pregnancy-enhanced vasodilator effects in the uterine arteries, mainly mediated by BKCa channels and sulfhydration.