The use of microbial siderophores for foliar iron application studies
The use of microbial siderophores for foliar iron application studies
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DOI：
10.1007/s11104-004-5212-2
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发表时间：
2005-05-01
期刊：
影响因子：
4.9
通讯作者：
Winkelmann, G
中科院分区：
文献类型：
--
作者：
Fernández, V;Ebert, G;Winkelmann, G
进行实验以使用微生物铁载体评估叶面施用的含铁化合物的分布。铁测定叶液离心获得根据铁螯合的测定方法的基础上，通过去铁胺E和反相柱上的HPLC分离。为了避免样品铁污染，治疗仅适用于一部分的叶后，一个系统的和可重复的程序和铁浓度专门确定从未处理的叶表面获得的流体。用蚕豆（Vicia faba L.）评价了叶液铁浓度的增加与叶施用的铁-铁载体、Fe-EDTA和FeSO 4 × 7 H（2）O的分布有关，Nicotiana tabacum L.和柑橘（Citrus madurensis Lour.）植物该方法可用于研究叶片铁渗透过程及其在植物体内的分布。本研究提供了叶面施用铁根铁蛋白、铁粪蛋白水解产物和铁二聚酸的渗透和分布的证据。
Experiments were conducted to assess the distribution of foliar applied Fe-containing compounds using microbial siderophores. Fe was measured in leaf fluid obtained by centrifugation according to a determination method based on Fe chelation by desferrioxamine E and HPLC separation on a reversed phase column. To avoid sample Fe contamination, treatments were only applied to a part of the leaf following a systematic and reproducible procedure and iron concentration was exclusively determined in fluid obtained from non-treated leaf surfaces. The increase in leaf fluid Fe concentration associated with the distribution of leaf applied Fe-siderophores, Fe-EDTA and FeSO4 x 7H(2)O was evaluated using Vicia faba L., Nicotiana tabacum L. and Citrus madurensis Lour. plants. The method proved useful to investigate the process of leaf Fe penetration and its distribution within the plant. Evidence of the penetration and distribution of leaf applied Fe-rhizoferrin, Fe-coprogen hydrolysis products and Fe-dimerum acid is presented in this study.