Establishment of a CRISPR/Cas9-mediated ANP32A homozygous knockout human embryonic stem cell line
Establishment of a CRISPR/Cas9-mediated ANP32A homozygous knockout human embryonic stem cell line
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CRISPR/Cas9介导的ANP32A纯合敲除人胚胎干细胞系的建立。
DOI：
10.1016/j.scr.2021.102234
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发表时间：
2021
影响因子：
1.2
通讯作者：
Shan Yongli✳
中科院分区：
文献类型：
--
作者：
Zhang Cong;Wei Yanxing;Zhang Yanqi;Zhang Jingyuan;Xing Qi;Zhou Min;Shan Yongli✳
ANP32A是酸性富亮氨酸核磷酸化蛋白32家族的成员，参与多种生化过程，包括染色质修饰和重塑。在此，我们建立了CRISPR/ cas9介导的anp32a纯合敲除人胚胎干细胞（ESC）系，以研究ANP32A在人胚胎干细胞多能性维持和分化过程中的作用。该细胞系具有正常的核型和典型的干细胞形态，具有高表达的多能基因和体外分化潜力。因此，ANP32A敲除细胞系为研究ANP32A在人类ESC细胞命运决定中的作用提供了一种有希望的方法。
ANP32A is a member of acidic leucine-rich nuclear phosphoprotein 32 family, which is involved in diverse biochemical processes, including chromatin modification and remodeling. Here, we established the CRISPR/Cas9-mediatedANP32Ahomozygous knockout human embryonic stem cell (ESC) line to investigate the roles of ANP32A in pluripotency maintenance and differentiation process of human ESCs. This cell line shows the normal karyotype and typical stem cell morphology, in accordance with high expression of pluripotent genes and the differentiation potentialin vitro. Consequently, the ANP32A knockout cell line provides a promising approach for investigating the roles of ANP32A in human ESC cell fate decisions.