Serum-free recombinant production of adenovirus using a hollow fiber capillary system
Serum-free recombinant production of adenovirus using a hollow fiber capillary system
复制标题
DOI：
10.2144/01302pf01
复制
发表时间：
2001-02-01
期刊：
影响因子：
2.7
通讯作者：
Kao, C
中科院分区：
文献类型：
--
作者：
Gardner, TA;Ko, SC;Kao, C
提出了一种新的生产腺病毒载体的方法，该方法的规模足以支持大多数研究应用和早期临床试验。该方法利用无血清细胞培养液和中空纤维细胞培养装置。与传统方法相比，所需的时间和空间要小得多，并且所得的腺病毒的体积要小得多，从而简化了纯化步骤。所描述的方法是一种可重复性的、方便的、生物安全的、对环境无害的用于实验室NSE的腺病毒载体的生产方法，并具有扩大临床使用的腺病毒生产的潜力。
A novel rnethod for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory nse and has the potential to scale-up the adenovirus production for clinical use.