On the prevalence of room-temperature protein phosphorescence.
On the prevalence of room-temperature protein phosphorescence.
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DOI：
10.1126/science.3576185
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发表时间：
1987-05
期刊：
影响因子：
56.9
通讯作者：
J. Vanderkooi;D. B. Calhoun;S. Englander
中科院分区：
文献类型：
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作者：
J. Vanderkooi;D. B. Calhoun;S. Englander
对大量蛋白质在室温脱氧水溶液中进行了本征磷光性质的测试。大多数蛋白质在正常溶液条件下表现出磷光。在四分之三的测试蛋白中观察到磷光寿命从0.5毫秒到2秒不等。寿命似乎与色氨酸吲哚侧链从溶剂中相对分离有关。除了少数例外，蛋白质通常可以预期显示大于30微秒的磷光寿命。蛋白质的这种普遍特性在很大程度上被忽视了，因为长时间的磷光对溶液中低水平溶解氧的猝灭高度敏感。蛋白质磷光为光致发光实验提供了一个新的时域和更宽的动态范围。
A large number of proteins were tested for the property of intrinsic phosphorescence in deoxygenated aqueous solution at room temperature. The majority of proteins exhibit phosphorescence under normal solution conditions. Phosphorescence lifetimes from 0.5 millisecond to 2 seconds were observed in three-fourths of the proteins tested. The lifetime appears to correlate with relative isolation of the tryptophan indole side chain from solvent. With few exceptions, proteins in general can be expected to display a phosphorescence lifetime greater than 30 microseconds. This widespread characteristic of proteins has been largely overlooked because long-lived phosphorescence is highly sensitive to quenching by low levels of dissolved oxygen in solution. Protein phosphorescence offers a new time domain and a far wider dynamic range than has been used before for photoluminescence experimentation.