Histopathological and behavioral characterization of a novel model of cardiac arrest and cardiopulmonary resuscitation in mice
Histopathological and behavioral characterization of a novel model of cardiac arrest and cardiopulmonary resuscitation in mice
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DOI：
10.1016/j.jneumeth.2003.12.024
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发表时间：
2004-06-15
影响因子：
3
通讯作者：
Traystman, RJ
中科院分区：
文献类型：
--
作者：
Kofler, J;Hattori, K;Traystman, RJ
心脏骤停与较高的死亡率和较差的神经预后有关。为了研究神经保护机制，我们对一种新的小鼠心脏骤停和心肺复苏(CPR)模型的功能和组织学结果进行了表征。用C57B1/6和129SVEV雄性小鼠静脉注射诱发心脏骤停。注射氯化钾。心脏停搏10min后，应用肾上腺素、100%氧气和胸外按压开始心肺复苏。在CPR前24小时和CPR后3天或7天，小鼠接受被动回避任务的行为测试、在开阔场地的运动活动和在T形迷宫中的自发交替。分别于心肺复苏后3天或7天测定大鼠海马区和尾壳核损伤程度。在这两个时间点，129SVEV小鼠的尾掌损伤较重。两个品系的缺血后小鼠在CPR后7d的T迷宫中正确选择次数减少，学习被动回避任务的能力暂时受损，在第3天有保留缺陷，但在第7天没有。运动能力表现出品系差异，C57B1/6小鼠更活跃，但与缺血相关的影响不大。功能和组织学结果之间的分离被发现，强调了结合两种结果测量对神经保护策略进行评估的重要性。(C)2004爱思唯尔B.V保留所有权利。
Cardiac arrest is associated with high mortality and poor neurological outcome. We characterized functional and histological outcome in a novel mouse model of cardiac arrest and cardiopulmonary resuscitation (CPR) in order to study neuroprotective mechanisms. Cardiac arrest was induced in male C57B1/6 and 129SVEV mice by i.v. injection of KCl. After 10 min cardiac standstill, CPR was initiated by administration of epinephrine, ventilation with 100% oxygen and chest compressions. Twenty-four hours before and 3 or 7 days after CPR, mice were subjected to behavioral testing using a passive avoidance task, locomotor activity in an open field, and spontaneous alternation in a T-maze. Hippocampal and caudoputamen injury was quantified 3 or 7 days after CPR. At both time points, caudoputamen injury was worse in 129SVEV mice. Post-ischemic mice of both strains showed a reduced number of correct choices in the T-maze up to 7 days after CPR, and were temporarily impaired in learning the passive avoidance task with a retention deficit on day 3 but not on day 7. Locomotor activity showed strain differences with C57B1/6 mice being more active, but little ischemia-related effects. A dissociation between functional and histological outcome was found emphasizing the importance of combining both outcome measures for evaluation of neuroprotective strategies. (C) 2004 Elsevier B.V All rights reserved.