The type II activin receptors are essential for egg cylinder growth, gastrulation, and rostral head development in mice.
The type II activin receptors are essential for egg cylinder growth, gastrulation, and rostral head development in mice.
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DOI：
10.1006/dbio.1999.9370
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发表时间：
1999-09
影响因子：
2.7
通讯作者：
Jihwan Song;Suk P. Oh;H. Schrewe;Masatoshi Nomura;Hong Lei;Makiko Okano;Thomas Gridley;En Li
中科院分区：
文献类型：
--
作者：
Jihwan Song;Suk P. Oh;H. Schrewe;Masatoshi Nomura;Hong Lei;Makiko Okano;Thomas Gridley;En Li
II型激活素受体ActRIIA和ActRIIB已被证明在小鼠的轴向模式和器官发育中起关键作用。为了研究它们的功能是否是非洲爪蟾研究中所涉及的中胚层形成和原肠胚形成所需的，我们通过杂交ActRIIA和ActRIIB敲除突变体产生了携带两种受体突变的小鼠。我们发现两种受体突变纯合的胚胎在卵柱期生长停滞，并且没有形成中胚层。进一步的分析显示，ActRIIA（-/-）ActRIIB（+/-）和约15%的ActRIIA（-/-）胚胎不能形成伸长的原条，导致胚胎中中胚层形成的严重破坏。有趣的是，我们在ActRIIA（-/-）nodal（+/-）双突变体中观察到类似的原肠胚形成缺陷，如果它们发展超过原肠胚形成阶段，则显示喙头缺陷和独眼畸形。这些结果提供了遗传学证据，II型激活素受体所需的卵柱生长，原条形成，和喙头发育小鼠。
The type II activin receptors, ActRIIA and ActRIIB, have been shown to play critical roles in axial patterning and organ development in mice. To investigate whether their function is required for mesoderm formation and gastrulation as implicated in Xenopus studies, we generated mice carrying both receptor mutations by interbreeding the ActRIIA and ActRIIB knockout mutants. We found that embryos homozygous for both receptor mutations were growth arrested at the egg cylinder stage and did not form mesoderm. Further analyses revealed that ActRIIA(-/-)ActRIIB(+/-) and about 15% of the ActRIIA(-/-) embryos failed to form an elongated primitive streak, resulting in severe disruption of mesoderm formation in the embryo proper. Interestingly, we observed similar gastrulation defects in ActRIIA(-/-)nodal(+/-) double mutants, which, if they developed beyond the gastrulation stage, displayed rostral head defects and cyclopia. These results provide genetic evidence that type II activin receptors are required for egg cylinder growth, primitive streak formation, and rostral head development in mice.