Human endometrial MAIT cells are transiently tissue resident and respond toNeisseria gonorrhoeae
Human endometrial MAIT cells are transiently tissue resident and respond toNeisseria gonorrhoeae
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DOI：
10.1038/s41385-020-0331-5
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发表时间：
2020-08-05
影响因子：
8
通讯作者：
Bjorkstrom, Niklas K.
中科院分区：
文献类型：
--
作者：
Bister, Jonna;Guterstam, Ylva Crona;Bjorkstrom, Niklas K.
粘膜相关不变性T细胞（MAIT）是非经典T细胞，在粘膜防御微生物中起重要作用。尽管人们对子宫内膜黏膜的免疫生物学越来越感兴趣，但对这个隔间中的人MAIT细胞知之甚少。MAIT细胞作为子宫内膜组织驻留的局部防御微生物的潜在作用在很大程度上尚未被探索。在这里，我们对绝经前和绝经后妇女子宫内膜以及妊娠早期蜕膜中的MAIT细胞进行了高维流式细胞术表征。此外，我们通过淋病奈瑟菌(neisseria gonorrhoeae， N。球菌)。子宫内膜MAIT （eMAIT）细胞代表了一个稳定的子宫内膜免疫细胞群，在月经周期、绝经后或对怀孕的反应中观察到有限的动态变化。此外，eMAIT细胞表现出活化的组织驻留表型。尽管eMAIT细胞表达CD69和CD103，但随着时间的推移，eMAIT细胞可以通过循环的MAIT细胞得到补充，以人类子宫移植为模型进行评估。最后，功能实验揭示了MAIT细胞对性传播和组织相关病原体N。球菌。总之，我们的研究为人类子宫内MAIT细胞动力学和抗微生物特性提供了新的见解。
Mucosa-associated invariant T (MAIT) cells are non-classical T cells important in the mucosal defense against microbes. Despite an increasing interest in the immunobiology of the endometrial mucosa, little is known regarding human MAIT cells in this compartment. The potential role of MAIT cells as a tissue-resident local defense against microbes in the endometrium is largely unexplored. Here, we performed a high-dimensional flow cytometry characterization of MAIT cells in endometrium from pre- and postmenopausal women, and in decidua from first-trimester pregnancies. Furthermore, we assessed MAIT cell function by stimulation withNeisseria gonorrhoeae(N. gonorrhoeae). Endometrial MAIT (eMAIT) cells represented a stable endometrial immune cell population as limited dynamic changes were observed during the menstrual cycle, post menopause, or in response to pregnancy. Furthermore, eMAIT cells exhibited an activated tissue-resident phenotype. Despite expressing CD69 and CD103, eMAIT cells were replenished over time by circulating MAIT cells, as assessed using human uterus transplantation as a model. Finally, functional experiments revealed the capability of MAIT cells to respond to the sexually transmitted and tissue-relevant pathogen,N. gonorrhoeae. In conclusion, our study provides novel insight into human MAIT cell dynamics and anti-microbial properties in the human uterus.