Low-Magnitude, High-Frequency Vibration Fails to Accelerate Ligament Healing but Stimulates Collagen Synthesis in the Achilles Tendon.
Low-Magnitude, High-Frequency Vibration Fails to Accelerate Ligament Healing but Stimulates Collagen Synthesis in the Achilles Tendon.
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DOI：
10.1177/2325967115585783
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发表时间：
2015-05
影响因子：
2.6
通讯作者：
Weinhold PS
中科院分区：
文献类型：
--
作者：
Thompson WR;Keller BV;Davis ML;Dahners LE;Weinhold PS
低震级、高频振动可加速骨折和伤口愈合，并防止肌肉骨骼组织的废弃萎缩。目的：探讨低强度高频振动对急性韧带损伤愈合的促进作用，并检测损伤肢体完整跟腱在低强度高频振动后的基因表达。对照实验室研究。将32只SD大鼠分为对照组和低震级高频振动组，行内侧副韧带全横断手术。从术后第2天开始小幅度高频振动，大鼠每天振动30分钟，连续12天。术后2周处死所有大鼠，对其完整和损伤的MCL进行生物力学测试或组织学分析。对损伤肢体的完整跟腱进行基因表达差异的评估。力学测试显示，无论是损伤的MCL还是完整的MCL，对照组和振动组的极限拉伸载荷或结构刚度没有差异。与对照组相比，振动暴露使完整跟腱中胶原1α(3倍)、白介素6(7倍)、环氧合酶2(5倍)和骨形态发生蛋白12(4倍)的基因表达增加(P<；0.05)。虽然在低强度、高频振动处理后，完全横断的MCL的力学或组织学特性没有观察到差异，但在完整的跟腱中观察到基因表达的显著增强。这些包括胶原蛋白、几种炎性细胞因子和对肌腱至关重要的生长因子。由于低幅度高频振动对韧带愈合无不良影响，振动疗法有望在不抑制韧带愈合的前提下，促进多发伤后其他组织(骨)的愈合。此外，完整跟腱在低幅度高频振动下基因表达增强，提示有必要进一步研究其在部分损伤或修复模型中加速肌腱愈合的潜力。
Low-magnitude, high-frequency vibration accelerates fracture and wound healing and prevents disuse atrophy in musculoskeletal tissues. To investigate the role of low-magnitude, high-frequency vibration as a treatment to accelerate healing of an acute ligament injury and to examine gene expression in the intact Achilles tendon of the injured limb after low-magnitude, high-frequency vibration. Controlled laboratory study. Complete surgical transection of the medial collateral ligament (MCL) was performed in 32 Sprague-Dawley rats, divided into control and low-magnitude, high-frequency vibration groups. Low-magnitude, high-frequency vibration started on postoperative day 2, and rats received vibration for 30 minutes a day for 12 days. All rats were sacrificed 2 weeks after the operation, and their intact and injured MCLs were biomechanically tested or used for histological analysis. Intact Achilles tendons from the injured limb were evaluated for differences in gene expression. Mechanical testing revealed no differences in the ultimate tensile load or the structural stiffness between the control and vibration groups for either the injured or intact MCL. Vibration exposure increased gene expression of collagen 1 alpha (3-fold), interleukin 6 (7-fold), cyclooxygenase 2 (5-fold), and bone morphogenetic protein 12 (4-fold) in the intact Achilles tendon when compared with control tendons (P < .05). While no differences were observed in the mechanical or histological properties of the fully transected MCL after low-magnitude, high-frequency vibration treatment, significant enhancements in gene expression were observed in the intact Achilles tendon. These included collagen, several inflammatory cytokines, and growth factors critical for tendons. As low-magnitude, high-frequency vibration had no negative effects on ligament healing, vibration therapy may be a useful tool to accelerate healing of other tissues (bone) in multitrauma injuries without inhibiting ligament healing. Additionally, the enhanced gene expression in response to low-magnitude, high-frequency vibration in the intact Achilles tendon suggests the need to further study its potential to accelerate tendon healing in partial injury or repair models.