The follow-up of progressive hypertrophic cardiomyopathy using magnetic resonance rotating frame relaxation times
The follow-up of progressive hypertrophic cardiomyopathy using magnetic resonance rotating frame relaxation times
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DOI：
10.1002/nbm.3871
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发表时间：
2018-02-01
影响因子：
2.9
通讯作者：
Liimatainen, Timo
中科院分区：
文献类型：
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作者：
Khan, Muhammad Arsalan;Laakso, Hanne;Liimatainen, Timo
磁共振旋转帧弛豫时间是诊断慢性心肌梗死的一种替代非造影剂选择。纤维化通常发生在进行性肥厚型心肌病中。使用旋转帧弛豫时间对心肌梗死组织中的纤维化进行成像，这为在没有造影剂的情况下随访进行性心肌病提供了可能性。用缩窄小鼠主动脉的方法造成轻度和重度左心室肥厚，分别于缩窄后第5、10、24、62和89天测定左心室纵向旋转坐标系的弛豫时间（T-1）和沿沿着虚拟场的弛豫时间（T-RAFF 2、T-RAFF 3）。采用Masson三色染色证实心肌纤维化。随着时间的推移，观察到纤维化组织和远端组织之间的T-1以及T-RAFF 2和T-RAFF 3的相对弛豫时间差增加。此外，T-RAFF 2和T-RAFF 3在纤维化组织中总体上显示出比T-1更高的弛豫时间。松弛时间差异与组织学证实的纤维化过度高度相关。我们发现T-RAFF 2和T-RAFF 3比T-1对肥厚性心肌病相关组织变化更敏感，可以作为非侵入性诊断磁共振成像标记物，用于随访进行性肥厚性心肌病小鼠模型。
Magnetic resonance rotating frame relaxation times are an alternative non-contrast agent choice for the diagnosis of chronic myocardial infarct. Fibrosis typically occurs in progressive hypertrophic cardiomyopathy. Fibrosis has been imaged in myocardial infarcted tissue using rotating frame relaxation times, which provides the possibility to follow up progressive cardiomyopathy without contrast agents. Mild and severe left ventricular hypertrophy were induced in mice by transverse aortic constriction, and the longitudinal rotating frame relaxation times (T-1) and relaxation along the fictitious field (T-RAFF2, T-RAFF3) were measured at 5, 10, 24, 62 and 89days after transverse aortic constriction in vivo. Myocardial fibrosis was verified using Masson's trichrome staining. Increases in the relative relaxation time differences of T-1, together with T-RAFF2 and T-RAFF3, between fibrotic and remote tissues over time were observed. Furthermore, T-RAFF2 and T-RAFF3 showed higher relaxation times overall in fibrotic tissue than T-1. Relaxation time differences were highly correlated with an excess of histologically verified fibrosis. We found that T-RAFF2 and T-RAFF3 are more sensitive than T-1 to hypertrophic cardiomyopathy-related tissue changes and can serve as non-invasive diagnostic magnetic resonance imaging markers to follow up the mouse model of progressive hypertrophic cardiomyopathy.