A novel hepatitis B vaccine containing Advax™, a polysaccharide adjuvant derived from delta inulin, induces robust humoral and cellular immunity with minimal reactogenicity in preclinical testing.
A novel hepatitis B vaccine containing Advax™, a polysaccharide adjuvant derived from delta inulin, induces robust humoral and cellular immunity with minimal reactogenicity in preclinical testing.
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DOI：
10.1016/j.vaccine.2012.12.077
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发表时间：
2013-04-08
期刊：
影响因子：
5.5
通讯作者：
Petrovsky, Nikolai
中科院分区：
文献类型：
--
作者：
Saade, Fadi;Honda-Okubo, Yoshikazu;Trec, Samay;Petrovsky, Nikolai
尽管目前的HBV疫苗具有突出的安全性和有效性记录，但免疫原性降低在老年人或患有肾损害或糖尿病的人中是一个问题。在这项研究中，我们测试了Advax™佐剂（一种基于δ菊粉的新型多糖佐剂）与传统明矾佐剂相比增强小鼠和豚鼠中乙型肝炎B表面抗原（HBs）免疫原性的能力。Advax™提供抗原保留，并显著增强抗-HBs抗体滴度和抗-HBs CD 4和CD 8 T细胞，增加Th 1、Th 2和Th 17细胞因子应答。与明矾不同，即使在HBs抗原之前24小时注射Advax™，也可观察到其佐剂作用。Advax™佐剂类似地增强豚鼠对第三代preS-HBs抗原的体液和细胞免疫应答。当与HBs抗原组合时，包含Advax™佐剂可以提供比当前一代HBV疫苗更强的保护，用于免疫低应答人群。
Although current HBV vaccines have an outstanding record of safety and efficacy, reduced immunogenicity is a problem in those of older age, or having renal impairment or diabetes mellitus. In this study, we tested the ability of Advax™ adjuvant, a novel polysaccharide adjuvant based on delta inulin, to enhance the immunogenicity of hepatitis B surface antigen (HBs) in mice and guinea pigs by comparison to the traditional alum adjuvant. Advax™ provided antigen-sparing and significantly enhanced both anti-HBs antibody titers and anti-HBs CD4 and CD8 T-cells, with increases in Th1, Th2 and Th17 cytokine responses. Unlike alum, the adjuvant effect of Advax™ was seen even when injected 24 hours before the HBs antigen. Advax™ adjuvant similarly enhanced humoral and cellular immune responses in guinea pigs to a third generation preS-HBs antigen. Inclusion Advax™ adjuvant when combined with HBs antigen could provide enhanced protection over current generation HBV vaccines for immunization of low responder populations.
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影响因子：
5.5
作者：
Gordon DL;Sajkov D;Woodman RJ;Honda-Okubo Y;Cox MM;Heinzel S;Petrovsky N
通讯作者：
Petrovsky N
影响因子：
15.9
作者：
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通讯作者：
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影响因子：
5.5
作者：
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通讯作者：
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影响因子：
5.5
作者：
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通讯作者：
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影响因子：
9.1
作者：
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通讯作者：
Heathcote, J