Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco
Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco
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DOI：
10.1186/s12879-019-4680-1
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发表时间：
2020-01-14
影响因子：
3.7
通讯作者：
Elmdaghri, Naima
中科院分区：
文献类型：
--
作者：
Katfy, Khalid;Diawara, Idrissa;Elmdaghri, Naima
背景资料：近几十年来，世界各地报告的百日咳病例数量显著增加，尽管儿童接种了有效的疫苗，但百日咳仍然是一种经常发生的疾病。本研究旨在确定卡萨布兰卡摩洛哥.Methods诊断为百日咳的儿童的家庭接触者的作用：从2015年11月至2017年10月，在卡萨布兰卡的Ibn Rochd大学医院咨询其家庭接触者的疑似百日咳儿童参加了本研究。对疑似患儿鼻咽部标本进行培养和RT-PCR分析。对家庭接触者，采集NP和血样，分别采用RT-PCR和ELISA法检测百日咳毒素抗体。结果：在研究期间，对128名疑似百日咳住院婴儿及其家属（N = 140）进行了调查。B。百日咳DNA特异性检出73例（57%），B共存。百日咳和B.副百日咳DNA阳性3例（2.3%），B共存。百日咳和B. 10份（7.81%）霍氏杆菌DNA和仅1份（0.78%）样品为IS 481 RT-PCR阳性，无法用所用诊断工具确定博德特氏菌属。通过培养、RT-PCR和血清学方法对家庭接触者百日咳感染的检出率分别为10%、46%和39%。在38%的病例中，在婴儿及其母亲中证实了百日咳DNA。B的共检测。百日咳和B.副百日咳DNA阳性率为2%，共检出B。百日咳和B. Holmesii DNA占4%。B。结论：本研究结果证实，B。百日咳仍然在儿童和成人中传播，可能是仍未接种疫苗的婴儿的百日咳污染源。采用B特异性RT-PCR。百日咳在成人诊断中的敏感性较低，应与血清学试验相结合，以提高百日咳的诊断水平，并有助于预防疾病在婴儿中的传播。
Background: In recent decades, there has been a marked increase in the number of reported cases of pertussis around the world, and pertussis continues to be a frequently occurring disease despite an effective childhood vaccination. This study aims to determine the role of household contacts of children diagnosed with pertussis in Casablanca Morocco.Methods: From November 2015 to October 2017, children suspected of whooping cough that consulted Ibn Rochd University hospital at Casablanca with their household contacts were enrolled in the study. Nasopharyngeal (NP) samples of the suspected children were analyzed by culture and RT-PCR. For the household contacts, NP and blood samples were collected and analyzed by RT-PCR and specific detection of pertussis toxin antibodies by ELISA, respectively.Results: During the study period, the survey was carried out on 128 infants hospitalized for pertussis suspicion and their families (N = 140). B. pertussis DNA was specifically detected in 73 (57%) samples, coexistence of B. pertussis and B. parapertussis DNA in 3 (2.3%) samples, coexistence of B. pertussis and B. holmesii DNA in 10 (7.81%) and only one (0.78%) sample was IS 481 RT-PCR positive without the possibility of determining the Bordetella species with the diagnostic tools used. Confirmations of Pertussis infection in household contacts by culture, RT-PCR and serology were 10, 46 and 39%, respectively.B. pertussis DNA was confirmed in the infants as well in their mothers in 38% of the cases. Co detection of B. pertussis and B. parapertussis DNA in 2% and co-detection of B. pertussis and B. holmesii DNA in 4%. B. holmesii DNA alone was detected in 5 NP samples of index cases and their mothers.Conclusions: The results of this study confirm that B. pertussis is still circulating in children and adults, and were likely a source of pertussis contamination in infants still not vaccinated. The use of RT-PCR specific for B. pertussis in the diagnosis of adults is less sensitive and should be associated with serologic tests to improve diagnosis of pertussis and contributes to preventing transmission of the disease in infants.