A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test
A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test
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DOI：
10.1016/s0022-1759(98)00136-7
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发表时间：
1998-10-01
影响因子：
2.2
通讯作者：
Davies, EG
中科院分区：
文献类型：
--
作者：
Richardson, MP;Ayliffe, MJ;Davies, EG
嗜中性粒细胞呼吸爆发对于宿主杀死摄入的微生物的能力是必不可少的。最近已经开发了几种流式细胞术测定来测量该过程。这些检测方法大多未经验证。在这项研究中，使用二氢罗丹明123（DHR）作为底物的全血流式细胞术测定与定量硝基四氮唑蓝（NBT）测试，呼吸爆发中的最早事件的公认措施进行了比较。由于使用全血，新的分析比现有的流式细胞术分析更快，更简单。可以使用小至0.1 ml的样本，这使得该测定非常适合用于新生儿和幼儿。DHR法与NET定量法之间有高度相关性（r（s）= 0.76，P < 0.01）。结论：全血DHR测定是呼吸爆发的准确和灵敏的措施。(C)1998 Elsevier Science B.V.保留所有权利。
The neutrophil respiratory burst is essential for the host's ability to kill ingested microorganisms. Several flow cytometric assays have recently been developed to measure this process. These assays are largely unvalidated. In this study a whole blood flow cytometry assay using dihydrorhodamine 123 (DHR) as a substrate was compared with the quantitative nitrobluetetrazolium (NBT) test, an accepted measure of the earliest events in the respiratory burst. Because whole blood is used, the new assay is quicker and simpler than existing flow cytometry assays. Specimens as small as 0.1 ml can be used which makes the assay ideal for use in neonates and young children. There was a high degree of correlation between the DHR assay and the quantitative NET test (r(s) = 0.76, P < 0.01). It is concluded that the whole blood DHR assay is an accurate and sensitive measure of the respiratory burst. (C) 1998 Elsevier Science B.V. All rights reserved.