CLASPs are CLIP-115 and-170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts
CLASPs are CLIP-115 and-170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts
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DOI：
10.1016/s0092-8674(01)00288-4
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发表时间：
2001-03-23
期刊：
影响因子：
64.5
通讯作者：
Galjart, N
中科院分区：
文献类型：
--
作者：
Akhmanova, A;Hoogenraad, CC;Galjart, N
CLIP-170和CLIP-115是细胞质连接蛋白，与生长中的微管末端特异性结合，并可作为抗突变因子。在这里，我们已经分离出两个CLIP相关蛋白（CLASPs），这是同源的果蝇轨道/桅杆微管相关蛋白。CLASP结合CLIP和微管，在微管远端与CLIP共定位，并在转染细胞中具有微管稳定作用。血清诱导后，CLASP重新定位于活动成纤维细胞前沿的微管远端段。我们提供的证据表明，这种不对称CLASP分布是由PI 3-激酶和GSK-3 β介导的。抗体注射表明CLASP 2是稳定微管朝向前缘的方向所必需的。我们建议CLASPs参与微管动力学响应位置线索的局部调节。
CLIP-170 and CLIP-115 are cytoplasmic linker proteins that associate specifically with the ends of growing microtubules and may act as anti-catastrophe factors. Here, we have isolated two CLIP-associated proteins (CLASPs), which are homologous to the Drosophila Orbit/Mast microtubule-associated protein. CLASPs bind CLIPs and microtubules, colocalize with the CLIPs at microtubule distal ends, and have microtubule-stabilizing effects in transfected cells. After serum induction, CLASPs relocalize to distal segments of microtubules at the leading edge of motile fibroblasts. We provide evidence that this asymmetric CLASP distribution is mediated by PI3-kinase and GSK-3 beta. Antibody injections suggest that CLASP2 is required for the orientation of stabilized microtubules toward the leading edge. We propose that CLASPs are involved in the local regulation of microtubule dynamics in response to positional cues.