Antioxidant, Antimicrobial, and Free Radical Scavenging Potential of Aerial Parts of Periploca aphylla and Ricinus communis.
Antioxidant, Antimicrobial, and Free Radical Scavenging Potential of Aerial Parts of Periploca aphylla and Ricinus communis.
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DOI：
10.5402/2012/563267
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Suleman S
中科院分区：
文献类型：
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作者：
Iqbal J;Zaib S;Farooq U;Khan A;Bibi I;Suleman S
语境。许多疾病与自由基引起的氧化应激有关。客观的。本研究评估了无叶杠和蓖麻地上部分的各种提取物的体外抗氧化和抗菌活性。材料和方法。采用DPPH和NO清除法测定植物提取物的体外抗氧化活性。通过与标准抗氧化剂相比硝基蓝四唑的还原来测量超氧阴离子自由基活性。分别采用没食子酸当量（GAE）法和连续管稀释法测定这些植物的总酚含量和抗菌活性。结果。植物表现出显着的自由基清除活性。结果以IC50表示。没食子酸正丙酯和 3-叔丁基-4-羟基苯甲醚用作抗氧化剂测定的标准品。两种植物的所有提取物均显示出与标准品相当的 IC50。植物提取物酚类含量高，抗菌活性与标准药物环丙沙星相当。讨论和结论。本研究提供的证据表明，无叶芦苇和蓖麻被证明是有效的天然抗氧化剂，可以替代合成抗氧化剂。植物还可以用来对抗致病菌株。
Context. Many diseases are associated with oxidative stress caused by free radicals. Objective. The present study evaluated the in vitro antioxidant and antibacterial activities of various extracts of aerial parts of Periploca aphylla and Ricinus communis. Materials and Methods. In vitro antioxidant activities of the plant extract were determined by DPPH and NO scavenging method. Superoxide anion radical activity was measured by the reduction of nitro blue tetrazolium as compared with standard antioxidants. Total phenolic contents and antibacterial activities of these plants were determined by gallic acid equivalent (GAE) and serial tube dilution method, respectively. Results. Plants showed significant radical scavenging activity. The results were expressed as IC50. n-Propyl gallate and 3-t-butyl-4-hydroxyanisole were used as standards for antioxidant assay. All the extracts of both plants showed comparable IC50 to those of standards. Plants extract exhibited high phenolic contents and antibacterial activities were comparable with standard drug, Ciprofloxacin. Discussion and Conclusion. The present study provides evidence that Periploca aphylla and Ricinus communis prove to be potent natural antioxidants and could replace synthetic antioxidants. Plants can also be used against pathogenic bacterial strains.