Glycosynthase-catalysed syntheses at pH below neutrality.
Glycosynthase-catalysed syntheses at pH below neutrality.
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pH 值低于中性时糖合酶催化的合成。
DOI：
10.1016/j.bmcl.2003.08.037
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发表时间：
2003
影响因子：
2.7
通讯作者：
M. Moracci
中科院分区：
文献类型：
--
作者：
A. Trincone;Assunta Giordano;G. Perugino;M. Rossi;M. Moracci
本文报道了利用新的糖合酶Ta-β- glye386g和已知的Ss-β- glye387g合成有趣的4-甲基伞形基双糖和4-戊烯-1-醇的α-和β-木糖的半乳糖化反应。结果表明，在低过量受体存在的情况下，反应收率令人满意，并表明这些酶在pH低于中性时的高活性适用于葡萄糖和半乳糖从首选的2- np基供体转移。
The use of the new glycosynthase Ta-β-GlyE386G and of the already known Ss-β-GlyE387G for the synthesis of interesting 4-methylumbelliferyl disaccharides and for the galactosylation of α- and β-xylosides of 4-penten-1-ol is reported. The results show satisfactory yields of reaction in presence of low excesses of acceptors and demonstrated that the high activity of these enzymes at pH below neutrality is applicable in the transfer of glucose as well as of galactose from the preferred 2-NP-based donors.