Genome-wide screens to identify genes of human pathogenic Yersinia species that are expressed during host infection.
Genome-wide screens to identify genes of human pathogenic Yersinia species that are expressed during host infection.
复制标题
DOI：
10.21775/cimb.007.135
复制
发表时间：
2005-07
影响因子：
3.1
通讯作者：
A. Darwin
中科院分区：
文献类型：
--
作者：
A. Darwin
研究引起人类疾病的细菌的一个明显目标是确定在宿主体内生长所需的细菌基因。从历史上看，这提出了重大的技术挑战。然而，带着这个目标，体内表达技术 (IVET) 和特征标记诱变 (STM) 技术在 20 世纪 90 年代被开发出来。这些技术已被用来鉴定三种人类致病性耶尔森氏菌（小肠结肠炎耶尔森氏菌、假结核耶尔森氏菌和鼠疫耶尔森氏菌）的毒力基因，并利用其小鼠感染模型的变化。在这篇综述中，每一项研究都被单独描述，包括每项研究如何完成的相关细节，以及对所识别基因的简要讨论。此外，还比较了这些 IVET 和 STM 筛选的结果，并讨论了所识别的基因之间明显缺乏重叠的情况。这些研究大多数是最近才发表的，这意味着对一些发现的新毒力基因的后续研究很少。然而，小肠结肠炎耶尔森氏菌 hreP、rscR 和 psp 基因已成为进一步研究的主题，在此也进行了总结。最后，我简要描述了使用全基因组（但不是体内）技术（消减杂交）来识别耶尔森氏菌毒力基因。
An obvious goal in the study of bacteria that cause human disease is to identify the bacterial genes required for growth within the host. Historically, this has presented a significant technological challenge. However, with this goal in mind, the in vivo expression technology (IVET) and signature-tagged mutagenesis (STM) techniques were developed during the 1990s. These techniques have been used to identify virulence genes in the three human pathogenic Yersinia species, Y. enterocolitica, Y. pseudotuberculosis and Y. pestis, using variations of their mouse models of infection. In this review, each of these studies is described individually, including the pertinent details of how each was done, and a brief discussion of the genes identified. In addition, the results of these IVET and STM screens are compared, and the striking lack of overlap between the genes identified is discussed. Most of these studies were only recently published, which means that there have been few follow-up studies on some of the novel virulence genes identified. However, the Y. enterocolitica hreP, rscR and psp genes have become the subject of further studies, which are also summarized here. Finally, I briefly describe the use of the genome-wide (but not in vivo) technology, subtractive hybridization, to identify Yersinia virulence genes.